ALI, N., AHMED, L., SHAHEIN, M. (2001). ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT. test, 49(3), 425-434. doi: 10.21608/vmjg.2001.372241
NAWAL M. ALI; LAMIA A AHMED; M SHAHEIN. "ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT". test, 49, 3, 2001, 425-434. doi: 10.21608/vmjg.2001.372241
ALI, N., AHMED, L., SHAHEIN, M. (2001). 'ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT', test, 49(3), pp. 425-434. doi: 10.21608/vmjg.2001.372241
ALI, N., AHMED, L., SHAHEIN, M. ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT. test, 2001; 49(3): 425-434. doi: 10.21608/vmjg.2001.372241

ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT

Veterinary Medical Journal (Giza)
Volume 49, Issue 3, July 2001, Pages 425-434    PDF (3.31 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2001.372241
Authors
NAWAL M. ALI* ; LAMIA A AHMED; M SHAHEIN
Department of Virology, Animal Health Research Institute, Dokki-Giza
Abstract
In summer 2000 several outbreaks among imported cattle with clinical manifestations similar to Bovine Ephemeral Fever (BEF) have been recorded in different Governorates in Egypt. 186 heparinized blood samples were collected for virus isolation and identification and 213 serum samples For serological examination. Suckling baby mice were intracerebrally (I/C) inoculated and Vero, BHK21 cells were also infected with buffy coat for viral isolation then indirect. Fluorescent antibody test (IFAT) was applied to Identify the BEF virus in impression smear of Baby mice brain and inoculated Vero and BHK21 Cells. So serological investigation was carried out to clear Up the immune status in infected cattle.
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