MADBOULY, H., KHASHABAH, E., IBRAHIM, N. (2001). PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.. test, 49(4), 563-573. doi: 10.21608/vmjg.2001.372275
H MADBOULY; ENSAF M KHASHABAH; NADIA M IBRAHIM. "PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.". test, 49, 4, 2001, 563-573. doi: 10.21608/vmjg.2001.372275
MADBOULY, H., KHASHABAH, E., IBRAHIM, N. (2001). 'PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.', test, 49(4), pp. 563-573. doi: 10.21608/vmjg.2001.372275
MADBOULY, H., KHASHABAH, E., IBRAHIM, N. PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.. test, 2001; 49(4): 563-573. doi: 10.21608/vmjg.2001.372275

PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.

Veterinary Medical Journal (Giza)
Volume 49, Issue 4, October 2001, Pages 563-573    PDF (3.65 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2001.372275
Authors
H MADBOULY* 1; ENSAF M KHASHABAH2; NADIA M IBRAHIM2
1Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch.
2Veterinary Serum and Vaccine Research Institute, Abbassia. Cairo.
Abstract
Very virulent IBDV was propagated for 3 passages in SPF-ECE followed by 3 passages in young susceptible chicks then for 3 passages in SPF. ECE then for further 12 passages in SPF-ECE. The first passage of last propagation was further propagated for 3 passages on Vero cells and for 60 passages in CEF-cell culture. The passage 40 of propagated IBDV proved to be safe and highly immunogenic by virus neutralization test. It still elicited high neutralizing antibody titers (18 log2) for a stationary phase about 7 months (the end of the experiment) and high values of lymphocyte blastogenesis (0.560 versus control 0.03). Vaccinated chicks well protected against challenge with highly virulent IBDV after 3 weeks post-vaccination (PV). The prepared vaccine has superior potential immunogenic effect than commercial live mild, and intermediate plus (hot) vaccines. The vaccine is effective even when preserved for 8 months (the end of ex- - periment) at -20°C.
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