RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. (2001). MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO. test, 49(4), 609-622. doi: 10.21608/vmjg.2001.372282
A RAMADAN; A ZAKI; H HASSAN; M HASHAD. "MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO". test, 49, 4, 2001, 609-622. doi: 10.21608/vmjg.2001.372282
RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. (2001). 'MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO', test, 49(4), pp. 609-622. doi: 10.21608/vmjg.2001.372282
RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO. test, 2001; 49(4): 609-622. doi: 10.21608/vmjg.2001.372282

MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO

Veterinary Medical Journal (Giza)
Volume 49, Issue 4, October 2001, Pages 609-622    PDF (4.88 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2001.372282
Authors
A RAMADAN* 1; A ZAKI1; H HASSAN2; M HASHAD2
1Immunobiology and Immunopharmacology Unit: Biology of Reproduction Department , Animal Reproduction Research Institute, P.O. Box 12556, Giza, Egypt
2Microbiology Dept. Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Abstract
Potential immunological effects of basic and acid- ic uterine luminal proteins (ULP) collected from estrus- and luteal-phase buffaloes were studied in vitro. The phagocytic and microbcidal activities of PMN and macrophages, nitric oxide production by macrophages, and superoxide anion production by PMN were elucidated. Estrus-phase basic and acidic ULP collected from buffaloes enhanced phagocytic activity of PMN at all concentrations tested, whereas basic and acidic ULP of luteal- phase buffaloes suppressed phagocytic activity in a dose-dependent manner. The bactericidal activ- ity of buffalo PMN increased in response to basic ULP of estrus-phase buffaloes in comparison with basic luteal-phase proteins. Meanwhile, acidic ULP proteins of estrus-phase buffaloes showed significant increase in bactericidal activity only at 40, 80, and 150 μg/ml protein concentrations in contrast to acidic ULP of luteal-phase buffaloes. Nitric oxide production by macrophages in- creased in response to basic ULP of estrus-phase buffaloes, whereas this activity decreased when macrophages were treated with different concentrations of basic ULP of luteal-phase buffaloes. Only at high concentrations the acidic protein of estrus-phase buffaloes increased nitric oxide production by macrophages as compared with acidic proteins from luteal-phase buffaloes. Superoxide anion productions by neutrophils increased in response to basic and acidic ULP of estrus-phase buffaloes and decreased in response to acidic pro- teins from luteal-phase buffaloes. These data demonstrate that buffalo uterus contains immunologically active secretory proteins that can influence the natural defense barriers of the buffalo genital tract.
Keywords
buffalo; uterine proteins; estrous cy- cle; phagocytic cells
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