M Atta, H., A El-Rehani, M., R Abdel Raheim, S. (2005). HYPERHOMOCYSTEINEMIA MODULATES VEGF PLASMA LEVELS AND ITS EXPRESSION IN LEUCOCYTES IN HEALTHY SUBJECTS AND IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE AND DIABETES MELLITUS. test, 24(2), 66-72. doi: 10.21608/ejsur.2005.373137
Hussein M Atta; Mahmoud A El-Rehani; Salama R Abdel Raheim. "HYPERHOMOCYSTEINEMIA MODULATES VEGF PLASMA LEVELS AND ITS EXPRESSION IN LEUCOCYTES IN HEALTHY SUBJECTS AND IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE AND DIABETES MELLITUS". test, 24, 2, 2005, 66-72. doi: 10.21608/ejsur.2005.373137
M Atta, H., A El-Rehani, M., R Abdel Raheim, S. (2005). 'HYPERHOMOCYSTEINEMIA MODULATES VEGF PLASMA LEVELS AND ITS EXPRESSION IN LEUCOCYTES IN HEALTHY SUBJECTS AND IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE AND DIABETES MELLITUS', test, 24(2), pp. 66-72. doi: 10.21608/ejsur.2005.373137
M Atta, H., A El-Rehani, M., R Abdel Raheim, S. HYPERHOMOCYSTEINEMIA MODULATES VEGF PLASMA LEVELS AND ITS EXPRESSION IN LEUCOCYTES IN HEALTHY SUBJECTS AND IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE AND DIABETES MELLITUS. test, 2005; 24(2): 66-72. doi: 10.21608/ejsur.2005.373137

HYPERHOMOCYSTEINEMIA MODULATES VEGF PLASMA LEVELS AND ITS EXPRESSION IN LEUCOCYTES IN HEALTHY SUBJECTS AND IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE AND DIABETES MELLITUS

The Egyptian Journal of Surgery
Volume 24, Issue 2, April 2005, Pages 66-72    PDF (218.54 K)
Document Type: Original Article
DOI: 10.21608/ejsur.2005.373137
Authors
Hussein M Atta* 1; Mahmoud A El-Rehani2; Salama R Abdel Raheim2
1Departments of Surgery, Faculty of Medicine, El-Minia University
2Biochemistry, Faculty of Medicine, El-Minia University
Abstract
Aim: Both homocysteine and vascular endothelial growth factor (VEGF) are implicated in development and progression of atherothrombotic vascular disease. We sought to determine whether there is a relationship between homocysteine and VEGF in healthy individuals and in patients with peripheral arterial disease (PAD) or diabetes mellitus (DM). 
Methods: Measurements of plasma homocysteine and VEGF and expression of VEGF in leucocytes were performed before and after intervention. Twelve healthy individuals were evaluated before and 4 h after methionine loading, whereas 10 patients with PAD and 15 patients with DM were evaluated before and 6 weeks after oral administration of B vitamins and folate. 
Results: Basal homocysteine was elevated in patients with PAD and DM (21.5±.9 and 19.9±1 µmol/l, respectively). Methionine resulted in significant elevation, while B vitamins resulted in significant reduction of homocysteine and VEGF and there was a significant correlation between homocysteine and VEGF changes (r = 0.73, p < 0.01). Moreover, VEGF mRNA expression in leucocytes was upregulated after methionine loading and was downregulated after B vitamins and folate treatment. 
Conclusion: These findings demonstrate that B vitamins and folate can successfully lower plasma homocysteine and VEGF expression in leucocytes in patients with PAD and DM. 
Keywords
Peripheral arterial disease; diabetes mellitus; homocysteine; vascular endothelial growth factor
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