THERAPEUTIC EFFECT OF ARTEMISIA ABSINTHIUM AND CURCUMA LONGA COMBINATION AGAINST CRYPTOSPORIDIOSIS IN IMMUNOSUPPRESSED MURINE MODEL | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 19, Volume 54, Issue 2, August 2024, Pages 325-334 PDF (2.51 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2024.373539 | ||
| Authors | ||
| NORAN M. ELDAWY1; AZZA SAAD ELGHAREEB2; HEBA E. ABDEL AATY3; IBRAHIM ALY4; NANCY O. KAMEL2 | ||
| 1Department of Medical Parasitology, Faculty of Medicine - Misr University for Science and Technology | ||
| 2Department of Medical Parasitology, Faculty of Medicine- October 6 University | ||
| 3Department of Medical Parasitology, Faculty of Medicine- Ain Shams University, 11566 Cairo and Armed Forces College of Medicine (AFCM), 11774 Cairo | ||
| 4Department of Medical Parasitology, Theodore Bilharz Research Institute, Giza, Egypt | ||
| Abstract | ||
| Infection by Cryptosporidium species causing cryptosporidiosis is considered a life-threatening condition in immunosuppressed patients, with unfortunately limited treatment options. This study evaluated the efficacy of Artemisia absinthium (AA) and Curcuma longa (CL) combination therapy unloaded and loaded on chitosan nanoparticles (CS NPs) and conjugated with conventional nitazo- xanide (NTZ) in treating cryptosporidiosis in immunosuppressed mice. The study design included fifty-six immunosuppressed mice classified into three main groups, divided into subgroups; eight mice each: GIA: non-infected non-treated, GIB: infected non-treated, GII: infected and treated with: CS NPs conventional free NTZ (GIIIA), AA and CL loaded on Cs NPs (GIIIB), conjugated AA and CL with NTZ loaded on Cs NPs (GIIIC), and AA and CL free combination (GIIID). Treatment efficacy was evaluated via parasitological examination, histopathological parameters, and the determination of oxidative stress markers. The most significant results were in the immun- osuppressed mice received unloaded unconjugated AA and CL free combination (GIIID) with a highly significant reduction (88%) in C. parvum oocysts shedding. Also, a remarked histo-patholo- gical improvement was revealed in the intestinal tissue sections nearly back to normal architecture and to lesser extent in extra-intestinal tissue sections in the same treated group. Meanwhile, oxidative stress markers (GSH & MDA) levels showed a high significant improvement (P< 0.001) in GIIID versus other treated ones. | ||
| Keywords | ||
| Mice; cryptosporidiosis; immunosuppressed; nitazoxanide; Artemisia absinthium; Cur-cuma longa; nanoparticles; CS NPs | ||
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