Inducing mutations using hydrazine hydrate, dimethyl sulfoxide and electric shock on three varieties of bread wheat (Triticum aestivum L.) | ||
| Archives of Agriculture Sciences Journal | ||
| Volume 6, Issue 3, December 2023, Pages 125-141 PDF (817.75 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/aasj.2023.374002 | ||
| Authors | ||
| H. G. SaKr* ; B. H. Ahmed | ||
| Agronomy Department, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt | ||
| Abstract | ||
| Three bread wheat varieties i.e, Shandawel 1, Sids 12 and Sakha 93 were subjected to three mutagenic agents i.e., hydrazine hydrate (HZ), at (1000, 2000 and 3000 ppm), of dimethyl sulfoxide at (1000, 2000 and 3000 ppm) and Electric shock at 220 V. in the presence of aqueous solution containing 10000, 20000 and 30000 ppm of sodium orthovanadate (Na3Vo4) to induce genetic variability and identify superior mutants for all the studded traits of each studied variety. One separate experiment was conducted for each variety during two successive growing seasons of 2021/2022 (M1) and 2022/2023 (M2) at the Experimental Farm, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt. The obtained results cleared that highly significant differences were detected among mutagenic agents for all the studded traits of each studied variety in the M2 generation. Superior mutated plants than control were detected for all the studded traits at all the mutagenic agents treatments of each variety in the M1 and M2 generations. Hydrazine hydrate was significantly the more effective mutagenic agents for inducing earliness in flowering for all studded varieties in the M1 and M2 generations, as well as dimethyl sulfoxide was for inducing other traits for most of studied varieties in both mutated generations. High broad sense heritability estimates were observed for most studied varieties in the M2 for all the studied traits of all the varieties, except 100 grain wheat of Sakha93, which was moderate. High expected gain from selection (GA) was detected in the M2 for grain yield/plant of all the studied varieties. | ||
| Keywords | ||
| Triticum aestivum; hydrazine hydrate; dimethyl sulfoxide; electric shock; heritability | ||
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