CHROMOSOME CONFIGURATION DURING IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 51, Issue 3, July 2003, Pages 411-420 PDF (2.46 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2003.375375 | ||
| Authors | ||
| KARIMA GH MAHMOUD* 1; K EL-SHAHAT2; W EL-NATTAT1 | ||
| 1Dept. of Animal Reproduction and A.L., National Research Center, Dokki. Egypt | ||
| 2Dept. of Theriogenology, Fac. Vet. Med., Cairo Univ., Egypt | ||
| Abstract | ||
| The aim of the present study was to examine the changes in chromosomal configuration of camel oocyte during in vitro maturation (IVM) at different culture periods of 24, 30, 36 and 48 hours and to compare the influence of pregnant camel serum and fetal calf serum on IVM of camel oocytes 24-30 hours. A total of 1978 oocytes were collected from 390 ovaries of slaughtered camels by aspiration method. The average number of camel oocytes per ovary was 5.30 ± 0.22. The proportion of COC, POC and DO oocytes was 29.27, 32.90 and 32.61% respectively. In addition, 5.21% of oocytes were degenerated. A high percentage of maturation was obtained at 24-30 hours (47.70% and 48.35% respectively). At 48 hours post-maturation, all metaphases were undefined due to degeneration of chromatin. it was found that, addition of pregnant camel serum had no beneficial effect on IVM of camel oocytes in comparison to fetal calf serum (40.10 vs 45.34% respectively). | ||
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