M, M., AFIFI, M. (1995). COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES. test, 43(1), 37-42. doi: 10.21608/vmjg.1995.375664
MADBOULY M; MANAL AFIFI. "COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES". test, 43, 1, 1995, 37-42. doi: 10.21608/vmjg.1995.375664
M, M., AFIFI, M. (1995). 'COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES', test, 43(1), pp. 37-42. doi: 10.21608/vmjg.1995.375664
M, M., AFIFI, M. COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES. test, 1995; 43(1): 37-42. doi: 10.21608/vmjg.1995.375664

COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES

Veterinary Medical Journal (Giza)
Volume 43, Issue 1, January 1995, Pages 37-42    PDF (3.89 M)
Document Type: Original Article
DOI: 10.21608/vmjg.1995.375664
Authors
MADBOULY M* 1; MANAL AFIFI2
1Ass. Prof. of virology, Fac. Vet. Med.; Beni-Suef, Cairo Univ.
2lecaturer of Poultry Dis., Fac. Vet.. Med; Cairo University.
Abstract
Forty-four bursal samples were collected from naturally infected and normal broiler chicks. The samples were homogenized and the homogenates were tested by agar gel precipitation test (AGPT), solid-phase ELISA (by indirect double antibodies sandwitch and single antibody ELISA systems) and Dot ELISA for detection of infectious bursal disease virus (IBDv) antigen. The reacted positive samples showed 86.3,88.6 and 88.6 percentage in AGPT, solid-phase ELISA and Dot ELISA, respectively. The findings suggest that the Dot ELISA is faster, more economic and easily applicable procedure than the other two techniques. The indirect double antibody sandwitch technique-is more sensitive than the indirect single antibody EIISA system.
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