MAHMOUD, N., SIAM, M. (1996). IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE. test, 44(3), 551-556. doi: 10.21608/vmjg.1996.376619
N. MAHMOUD; M. SIAM. "IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE". test, 44, 3, 1996, 551-556. doi: 10.21608/vmjg.1996.376619
MAHMOUD, N., SIAM, M. (1996). 'IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE', test, 44(3), pp. 551-556. doi: 10.21608/vmjg.1996.376619
MAHMOUD, N., SIAM, M. IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE. test, 1996; 44(3): 551-556. doi: 10.21608/vmjg.1996.376619

IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE

Veterinary Medical Journal (Giza)
Volume 44, Issue 3, July 1996, Pages 551-556    PDF (3.79 M)
Document Type: Original Article
DOI: 10.21608/vmjg.1996.376619
Authors
N. MAHMOUD* 1; M. SIAM2
1Fish Diseases Department, Animal Health Research Institute, Dokki, Giza, Egypt.
2Faculty of veterinary Medicine, Cairo Univ.
Abstract
Amplicaecum larvae are a significant parasite infesting Tilapia and some predatory fish species at Nasser Lake since 1988. these laval stages were homogenized and prepared as a crude antigen (structural antigen). The protein concentration was estimated by monitoring the UV absorption Spectrum. The positive immune serum anti Amplicaecum larval haemoglobin was prepared and sensitiized in vivo. The highest sensitivity of the haemoglobin was estimated by ELISA technique and amounted to a titer of 1/20 with optical density x 0.554.
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