. NAWWAR, A., MOUSA, H., AMIN, D., ABD. EL -AZIZ, T. (1996). STUDIES ON RAPID DIAGNOSTIC METHODS FOR RAC 33IT HAEMORRHAGIC VIRAL DISEASE (RHVD). test, 44(3), 601-611. doi: 10.21608/vmjg.1996.376627
AMINA . NAWWAR; HADIA MOUSA; DAWLAT AMIN; TIIAN ABD. EL -AZIZ. "STUDIES ON RAPID DIAGNOSTIC METHODS FOR RAC 33IT HAEMORRHAGIC VIRAL DISEASE (RHVD)". test, 44, 3, 1996, 601-611. doi: 10.21608/vmjg.1996.376627
. NAWWAR, A., MOUSA, H., AMIN, D., ABD. EL -AZIZ, T. (1996). 'STUDIES ON RAPID DIAGNOSTIC METHODS FOR RAC 33IT HAEMORRHAGIC VIRAL DISEASE (RHVD)', test, 44(3), pp. 601-611. doi: 10.21608/vmjg.1996.376627
. NAWWAR, A., MOUSA, H., AMIN, D., ABD. EL -AZIZ, T. STUDIES ON RAPID DIAGNOSTIC METHODS FOR RAC 33IT HAEMORRHAGIC VIRAL DISEASE (RHVD). test, 1996; 44(3): 601-611. doi: 10.21608/vmjg.1996.376627

STUDIES ON RAPID DIAGNOSTIC METHODS FOR RAC 33IT HAEMORRHAGIC VIRAL DISEASE (RHVD)

Veterinary Medical Journal (Giza)
Volume 44, Issue 3, July 1996, Pages 601-611    PDF (6.99 M)
DOI: 10.21608/vmjg.1996.376627
Authors
AMINA . NAWWAR* ; HADIA MOUSA; DAWLAT AMIN; TIIAN ABD. EL -AZIZ
Animal Health Research Institute
Abstract
Rabbit haemorrhagic disease virus (local isolate) was successfully propagated in MDBK cell line culture. The RHVD was detected in organs of naturally infected rabbits using AGP, HA, ELISA, IP and IFA tests. 
Infected liver homogenate gave the highest absorbance value (2.439) than other organs. Immune peroxidase staining and indirect immunofluorescence showed the specific RHVD reaction more intense in liver cells. AGP test was considered less sensitive in the detection of the RHVD in infected organ homogenates. ELISA, IP and IFA were rapid sensitive, specific tests for diagnosis of RHVD directly from naturally infected rabbits.
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