Light and electron microscopic study on the effect of immobilization stress on adrenal cortex of adult rats and possible ameliorative role of vitamin E. | ||
| Journal of Medical Histology | ||
| Article 5, Volume 1, Issue 1, June 2017, Pages 44-56 PDF (6.81 M) | ||
| Document Type: Review article | ||
| DOI: 10.21608/jmh.2017.961.1012 | ||
| Authors | ||
| Zainab Altayeb1; Maysara Salem2 | ||
| 1Histology and Cell Biology Department, Faculty of Medicine, Helwan University | ||
| 2Histology and Cell Biology Department, Faculty of Medicine, Benha University | ||
| Abstract | ||
| Background: Modern society is full of stress. Immobilization stress is a model used in animals to study its effects. Vitamin E is an antioxidant that protects biological membranes from oxidative stress. Objectives: This study aimed to investigate the effects of immobilization stress on the adrenal cortex of rats and the ameliorative effect of vitamin E. Materials and Methods: Forty adult male rats were used in this study and were divided equally into 4 groups. Group I: were divided equally into negative and positive controls. Group II: rats received vitamin E at a dose of 40 I.U/kg by gastric tube for 30 days. Group III: rats subjected to immobilization stress 2 hours /day for 30 days. Group IV: rats subjected to immobilization stress 2 hours /day and received 40 I.U/kg of vitamin E for 30 days. Adrenal sections were histologically prepared and examined. Results: Group II was comparable to group IA. Group III revealed zona glomerulosa (ZG) with loss of normal architecture, zona fasciculata (ZF) with multiple cells containing cytoplasmic vacuolations and darkly stained nuclei. By EM ZG cells appeared with accumulations of lipid droplets and lysosomes, irregular nuclear envelopes and chromatin condensation. ZF cells showed numerous lipid droplets and irregular nuclear envelopes. There were significant increases in the mean area of collagen fibers and mean serum cortisol level. Group IV revealed regression of these changes and increase in the mean count of CD44 +ve cells.Conclusions: Immobilization stress exerted deleterious suprarenal cortical changes and vitamin E had an ameliorative effect. | ||
| Keywords | ||
| adrenal cortex; Immobilization stress; suprarenal gland; Vitamin E | ||
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