CHARACTERIZATION OF ANTIGENIC PROPERTY OF FASCIOLA HEPATICA AND FASCIOLA GIGANTICA | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 46, Issue 4, October 1998, Pages 507-516 PDF (3.19 M) | ||
| DOI: 10.21608/vmjg.1998.377334 | ||
| Authors | ||
| SAHLAB A.A.M.* 1; ABDEL-AAL A.A.2 | ||
| 1Animal Health Research Institute . Devices and Biotechnology unit. | ||
| 2Department of Parasitology Faculty of Vet. Med. Suez canal Univ. | ||
| Abstract | ||
| Differential molecular studies was performed between Fasciola hepatica and Fasciola gigantica. The total protein content of whole F. hepatica antigen (85mg/gm) seemed to be double value of F.gigantica (42mg/gm) while the excretory secretory product of F. hepatica and F. gigantica was 60 and 52mg/gm respectively. SDS-PAGE of both whole and excretory-secretory antigen of F.hepatica as well as F. gigantica showed common bands at 14.5kd. and 28kd. and each one had characteristic bands Clustered in different molecular weights. The western blot showed cross reactive immune bands at 14.5kd. and 28 kd. while 47kd. was specific to F. hepatica but the bands located at 57kd, 69kd, 88kd were specific to F. gigatica. The western blot was more sensitive and can predict infected case than abattoir post mortem examination. The work can be used to determine the early incidence of fascioliasis including Fasciola Species. | ||
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