Evaluation of Immunization with Native NetB in Addition to Reactive Proteins Against Necrotic Enteritis in Broiler Chickens | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 01 September 2024 PDF (1.79 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.297718.2175 | ||
| Authors | ||
| Nashwa M.Eid Elsayed* 1; Eman Fathy Farag2; Salama Aboohmra shany1; Al-Hussien Mohamed Dahshan1; Ahmed Ali Mohamed1 | ||
| 11Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt | ||
| 2animal health research institute | ||
| Abstract | ||
| Clostridium perfringens (CP) induces necrotic enteritis (NE) and has re-emerged as one of the deadliest diseases in the poultry industry after banning antibiotics in poultry feed. The study aimed to prepare and assess the protective potential of four native CP type A vaccines against necrotic enteritis (NE) in commercial broiler chickens; three are NetB +ve including; formalized toxoid (FTNB), sonicated supernatant (SSNB), boiled bacterin (BBNB) and the fourth is NetB -ve (formalized toxoid (FT). SSNB, intracytoplasmic proteins; perfringolysin O, glyceraldehyde-3-phosphate dehydrogenase (GPD), and fructose 1,6-biphosphate aldolase(FBA) while BBNB, cell wall proteins; hypothetical proteins (HPs), pyruvate ferredoxin oxidoreductase (PFOR), elongation factor-Tu (EF-TU), and collagen adhesion protein (cnaA). SSNB and BBNB proteins were electro-phoretically divided. Western blotting was done to determine their capacity to elicit an immunological response against NE. 120 one-day-old broiler chicks had been categorized into six groups; control negative(C-ve), FTNB, SSNB, BBNB, FT, and control positive (C+ve). Vaccines were mixed with Montanide™ ISA 71 VG adjuvant. Chicks are vaccinated subcutaneously at 3 and 9 days. Orally challenged groups with Eimeria maxima on day 20 and CP at 24 to 26 days of age. Mortality was observed within 3 days post-challenge in the control positive group only (25%). Higher IgY antibody ELISA titers are found in SSNB and BBNB practically than FT group. The expression of a gene of interleukin-17 (IL-17) in spleen tissue was downregulated in all groups except FT and C+ve groups. These show the efficiency of native NetB with other protective component antigens against NE in broilers. | ||
| Keywords | ||
| Clostridium perfringens; ELISA; Interlukien-17; Necrotic enteritis; Vaccine | ||
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