ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. (2024). Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat. test, 2(2), 157-194. doi: 10.21608/ejctr.2024.283521.1015
Asmaa Mohamed ElHeety; Esam Mohammed Elshwaihy; Thoraya Abd El-Aziz El-deeb; Maram Mohamed Elkelany. "Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat". test, 2, 2, 2024, 157-194. doi: 10.21608/ejctr.2024.283521.1015
ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. (2024). 'Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat', test, 2(2), pp. 157-194. doi: 10.21608/ejctr.2024.283521.1015
ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat. test, 2024; 2(2): 157-194. doi: 10.21608/ejctr.2024.283521.1015

Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat

Egyptian Journal of Cell and Tissue Research
Volume 2, Issue 2, June 2024, Pages 157-194    PDF (4.84 M)
Document Type: Original articles
DOI: 10.21608/ejctr.2024.283521.1015
Authors
Asmaa Mohamed ElHeety1; Esam Mohammed Elshwaihy2; Thoraya Abd El-Aziz El-deeb3; Maram Mohamed Elkelany* 4
1Demonstrator of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
2Assistant Professor of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
3Professor of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
4Lecturer of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
Abstract
Background: Cyclophosphamide (CYP) is an alkylating drug used in cancer treatment, one of its common complications is hemorrhagic cystitis (HC). Pumpkin seed oil (PSO) is a strong anti-inflammatory, antioxidant and anticancer. Aim of the Work: This study was carried out to study the possible protective effect of pumpkin seed oil on cyclophosphamide induced hemorrhagic cystitis. Materials & Methods: The present study was carried out on 45 adult male albino rats. They were randomly divided into four main groups. Group I represented the control group. Group ll (PSO) received PSO at a dose of (40mg/kg/day) orally for 28 days. Group lll (HC) received CYP solution (75 mg/kg) for induction of HC. Group IV (PSO+HC) received PSO for 28 days as in group II, and at the 20th day, HC was induced by the same dose and manner as in group III. The urinary bladder specimens were processed for different histological techniques. Morphometrical and statistical studies were also performed. Results: Group III showed a lot of changes at the level of light microscopic study including epithelial ulceration, nuclear changes, hemorrhage, extensive fibrosis in Masson Trichrome stained sections and weak PAS reaction. Furthermore, HC group showed strong positive immune reaction in the Bax immunostained sections. These findings were confirmed in our morphometric data. Additionally, ultrastructural examination of HC group showed rarefied cytoplasm, widening of intercellular spaces and swollen mitochondria. Meanwhile, group IV showed marked improvement of the previously mentioned pathological changes. Conclusion: PSO administration plays a protective role in cyclophosphamide-induced hemorrhagic cystitis.
Keywords
Cyclophosphamide; PSO; urinary bladder; Bax; ultrastructure
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