Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt | ||
| Parasitologists United Journal | ||
| Article 7, Volume 17, Issue 2, August 2024, Pages 121-125 PDF (423.98 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/puj.2024.297600.1252 | ||
| Authors | ||
| Asmaa Abd Ellah* ; Manal GabAllah | ||
| Department of Medical Parasitology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||
| Abstract | ||
| Background: The prevalence of bovine sarcocystosis is high in most regions of the world. In Egypt, it constitutes a major human health problem due to consumption of insufficiently cooked bovine meat. Objective: To determine the detection rate and species identity of Sarcocystis infection in slaughtered water buffaloes (Bubalus bubalis) in Egypt. Material and Methods: Samples from esophagus and tongue were collected from 100 slaughtered buffaloes from different slaughterhouses in Sohag, Egypt and subjected to macroscopical, microscopical, and histopathological examinations. The genomic DNA was extracted from Sarcocystis-infected muscle samples and PCR–RFLP method was used to amplify partial parts of the gene encoding 18S rRNA. The PCR products were digested by restriction endonuclease enzyme BclI for species identification. Results: Macroscopic sarcocysts were detected in 25% of slaughtered buffaloes, however, no microscopic sarcocysts were detected. Molecular analysis resulted in successful amplification of all positive samples and S. fusiformis was identified in all positive samples. Conclusion: All isolates obtained from slaughtered buffaloes in Sohag, Egypt were S. fusiformis suggesting the potential role of cats in the transmission of S. fusiformis. Our study recommends strict hygienic measures to protect animals and humans from infection. | ||
| Keywords | ||
| 18S rRNA; bovine carcasses; Egypt; PCR-RFLP; Sarcocystis spp; S. fusiformis; Sohag; water buffaloes | ||
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