IBRAHIM, H., HANAFI, M., ELBIALY, A., E, H. (2006). STUDIES ON TRANSFORMATION OF RESISTANT PLASMID BETWEEN SALMONELLA AND E. COLI. test, 54(4), 777-786. doi: 10.21608/vmjg.2006.379904
HANAN IBRAHIM; MERVAT HANAFI; AMANY ELBIALY; HATEM E. "STUDIES ON TRANSFORMATION OF RESISTANT PLASMID BETWEEN SALMONELLA AND E. COLI". test, 54, 4, 2006, 777-786. doi: 10.21608/vmjg.2006.379904
IBRAHIM, H., HANAFI, M., ELBIALY, A., E, H. (2006). 'STUDIES ON TRANSFORMATION OF RESISTANT PLASMID BETWEEN SALMONELLA AND E. COLI', test, 54(4), pp. 777-786. doi: 10.21608/vmjg.2006.379904
IBRAHIM, H., HANAFI, M., ELBIALY, A., E, H. STUDIES ON TRANSFORMATION OF RESISTANT PLASMID BETWEEN SALMONELLA AND E. COLI. test, 2006; 54(4): 777-786. doi: 10.21608/vmjg.2006.379904

STUDIES ON TRANSFORMATION OF RESISTANT PLASMID BETWEEN SALMONELLA AND E. COLI

Veterinary Medical Journal (Giza)
Volume 54, Issue 4, October 2006, Pages 777-786    PDF (2.25 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2006.379904
Authors
HANAN IBRAHIM* 1; MERVAT HANAFI1; AMANY ELBIALY1; HATEM E2
1Serology Unit, Animal Health Research Institute, Dokki, Egypt.
2Microbiology of Department, Faculty. Vet. Med., Cairo University
Abstract
Plasmid profile analysis of 10 multiple drug-resistant Salmonella serovars with (resistant to ampicillin, chloramphenicol, oxytetracycline, nalidixic acid and streptomycin) was performed using agarose gel electrophoresis. There were 8 isolates harboring plasmid with molecular weight ranged from 16-31.5kpb while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S.Kentucky and S.Typhimurium as donor bacteria and E.coli XLl as recipient bacteria by electroporation. Transconjugants were obtained by conjugation assay between multiple drug resistant (MDR) S.Typhimurium as donor bacteria and each of S.Entritidis, S.Typhi, E.coli: XLl and E.coli:O157H7 as recipient bacteria.
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