DETECTION OF CORONAVIRUS ANTIGEN IN FAECAL SAMPLES OBTATNES FROM NEWBORN BUFFALOE CALVES . | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 38, Issue 1, January 1990, Pages 77-85 PDF (4.13 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.1990.380153 | ||
| Authors | ||
| A. KARIM1; M . YOUSEF1; A. NAWAL2 | ||
| 1Faculty of Veterinary Medicine, Cairo University Giza, Egypt. | ||
| 2Animal Heath Research Institute, Dokki. Giza, Egypt. | ||
| Abstract | ||
| Diarrhea remains one of the most important causes of calf mortality. An attempt has been made to gather current relevant clinical information relating to the aetiology of calf enteritis especially that of | viral origin. The association between coronavirus infection and diarrhea in calves was studied by Mebus and Coworkers (1973 a and 1973 b). Methods of diagnosing coronaviral infections of cal- ves were studied clinically and pathologically (Stair et al., 1972; and Marin et al., 1974). Virological examination for coronavirus in calves Was studied by Patel et al. (1982), where the virus was detected by indirect immunofluorescence and electron microscopy. in this work, three different methods were used for detection of coronavirus antigen(s) in faecal samples of newborn Egyptian buffalo calves suffering from diarrhea .these were the Enzyme Linked Immunosorbent Assay (ELISA), the Antigen Sopt Test (AST) and the immunoperoxidase (IP) technique. | ||
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