EL-Sahrigy, S., Abdel Gawad, T., Abdel Rahman, A., Magdy, S., Mostafa, E., Hasanin, R., Galal, A., Hamed, H., Hassan, E., Abdelrahman, A., Hassan, M. (2024). Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome. test, 33(4), 211-219. doi: 10.21608/ejmm.2024.319256.1334
Sally A. F. EL-Sahrigy; Tarek A. Abdel Gawad; Azza M. O, Abdel Rahman; Sondos Magdy; Eman A. Mostafa; Rasha M. Hasanin; Ashraf Galal; Hanan M. Hamed; Eman M. Hassan; Amany H. Abdelrahman; Mirhane Hassan. "Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome". test, 33, 4, 2024, 211-219. doi: 10.21608/ejmm.2024.319256.1334
EL-Sahrigy, S., Abdel Gawad, T., Abdel Rahman, A., Magdy, S., Mostafa, E., Hasanin, R., Galal, A., Hamed, H., Hassan, E., Abdelrahman, A., Hassan, M. (2024). 'Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome', test, 33(4), pp. 211-219. doi: 10.21608/ejmm.2024.319256.1334
EL-Sahrigy, S., Abdel Gawad, T., Abdel Rahman, A., Magdy, S., Mostafa, E., Hasanin, R., Galal, A., Hamed, H., Hassan, E., Abdelrahman, A., Hassan, M. Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome. test, 2024; 33(4): 211-219. doi: 10.21608/ejmm.2024.319256.1334

Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome

Egyptian Journal of Medical Microbiology
Article 26, Volume 33, Issue 4, October 2024, Pages 211-219    PDF (517.41 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.319256.1334
Authors
Sally A. F. EL-Sahrigy1; Tarek A. Abdel Gawad2; Azza M. O, Abdel Rahman1; Sondos Magdy2; Eman A. Mostafa1; Rasha M. Hasanin1; Ashraf Galal1; Hanan M. Hamed1; Eman M. Hassan3; Amany H. Abdelrahman3; Mirhane Hassan* 3
1Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt
2Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
3Clinical and Chemical Pathology Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt
Abstract
Background: Sepsis is a life- threatening organ dysfunction caused by impaired host response to infection. Objectives: To assess the immune status in children with sepsis and septic shock using mRNA gene expression of 7 biomarkers; Interleukin (IL)-1ß, Tumor necrosis factor (TNF), IL-10, Programmed death protein (PD-1) and Programmed death ligand 1(PD-L1), BID (inhibitory check point), and BCL2 (anti-apoptotic). Secondly, to study the possible influence of G-CSF on the expression of these biomarkers and outcome in septic children. Methodology: This is a case controlled prospective randomized biomarker study including 65 subjects divided into 3 groups. Group 1 comprised 24 septic patients receiving G-CSF in addition to conventional treatment for sepsis. Group 2 included 17 septic patients on conventional treatment. Group 3 control group comprised 24 infants and children matched for age and sex. Two blood samples were withdrawn from each patient at day 1 and day 8. RT-PCR analysis for mRNA gene expression of the studied biomarkers were performed. Results: Group 1 showed significant increase of PD-L1 on day 8 compared to day 1 (p= 0.019) and significant increase of BID1 on day 8 (p= 0.014) and on day 1 (p= 0.08) compared to healthy children. In group 2, IL-10 showed significant decrease on day 8 compared to day 1(p= 0.02). BID1 showed highly significant increase between both days 1 and 8 versus healthy children (p = 0.001, 0.004 respectively). BCL-2 showed significant decrease in serum levels between both days 1 and 8 versus healthy children (p = 0.04 ,0.012 respectively). Conclusion: Upregulation of the apoptotic BID1 and PD-L1 expression with downregulation of IL-10 and BcL2 expression in our septic patients suggests a feature of sepsis-related immunosuppression. G-CSF had no role on the studied biomarkers release and outcome.
Keywords
apoptotic BID1; PD-L1; BCL2; cytokines; sepsis
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