Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythematosus and Lupus Nephritis | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 27, Volume 33, Issue 4, October 2024, Pages 221-226 PDF (333.01 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2024.318532.1330 | ||
| Authors | ||
| Ingy Ashmawy* 1; Abeer Ramadan2; Neveen A. Helmy1; Huda Marzouk3; Yomna Farag3; Shereen H. Abd El Aziz1 | ||
| 1Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt | ||
| 2Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Institute, National Research Centre, Cairo, Egypt | ||
| 3Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| Abstract | ||
| Background: Juvenile onset systemic lupus erythematous (JSLE) and the accompanying lupus nephritis (LN) (affects up to 80% of JSLE) harbor more aggressive disease courses and major complications with higher morbidity and mortality risks compared to adult onset SLE. Two microRNAs (miRNAs), miR-130b-3p and miR-148a-3p, are strong candidates in the pathogenesis of both JSLE and LN. Objective: Studying serum expression of miR-130b-3p and miR-148a-3p patients with JSLE and LN to evaluate their contribution to their pathogenesis. Methodology: The study included 100 LN, 50 JSLE and 50 healthy controls (HC), reverse transcriptase real time PCR (RT-QPCR) was used for evaluation of serum levels of miR-130b-3p and miR-148a-3p. Results: miR-130b-3p showed decreased expression in JSLE and LN patients compared to HC and correlated inversely with serum creatinine within patients’ groups. miR-148a-3p significantly decreased expression in JSLE patients compared to controls and LN patients. MiR-148a-3p was elevated in LN cases compared to JSLE but not HC showing inverse correlation with duration of LN and serum creatinine. Positive correlation between miR-130b-3p and miR-148a-3p was detected in diseased groups (r = 0.75, p = 0.001). Conclusion: Our study was the first to analyze both miR-130b-3p & miR-148a-3p expression in JSLE and LN and we concluded that both might affect the pathogenesis of JSLE and that miR-148a-3p may significantly contribute to the pathogenesis of LN among JSLE patients. | ||
| Keywords | ||
| Keywords: SLE; Juvenile onset systemic lupus erythematous; Lupus nephritis; miR-130b-3p; miR-148a-3p | ||
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