MicroRNA-145 Inhibitor Induced Proliferation of Human Oral Squamous Cell Carcinoma by Upregulating C-Myc and Downregulating Caspase-3 Genes: An In-Vitro Study | ||
| Egyptian Dental Journal | ||
| Volume 70, Issue 4 - Serial Number 3, October 2024, Pages 3305-3316 PDF (1.37 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2024.302310.3107 | ||
| Authors | ||
| Noran Ahmed Salem* 1; Sherif Faroq Elgayar2; Maii Ibrahim Sholqamy3; Abd El Rahman M Sharfeldeen4; Sabreen Gamal Khalil Amar5 | ||
| 1Demonstrator of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Egypt. | ||
| 2Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt. | ||
| 3Associate Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt | ||
| 4Lecturer of oral and maxillofacial pathology, Faculty of Dentistry, Assiut university, Egypt, drabdelrahman12@aun.edu.eg Assistant Professor of oral pathology, collage of dentistry, City University Ajman, Ajman, UAE, a.abdel@cu.ac.ae | ||
| 5Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt. | ||
| Abstract | ||
| Background: Oral cancer has contributed to a tremendous death rate and the total survival rate is expected to be about 50% after 5 years. MicroRNAs are associated with oral carcinogenesis due to their ability to regulate gene expression. To limit tumor growth, miR-145 influences signaling pathways by targeting tumor-specific genes. Objective: This research was conducted to investigate the potential impact of miR-145 on the human oral squamous cell carcinoma cell line (OECM-1), and Human Oral Fibroblast cell line (HOrF) as negative control cells. Also, to investigate its impact on the expression of C-Myc and Caspase-3 genes. Material and Methods: In this study, there were four groups. Group I: untreated OECM-1, Group II: treated OECM-1, Group III: Untreated HOrF, and Group IV: treated HOrF. MiR-145 inhibitor was transfected into cell lines. Methyl Thiazole Tetrazolium assay was used to analyze the vitality and oralthe cell proliferation rate of the cell lines. Furthermore, the expression of C-Myc, and Caspase-3 was assessed in OECM-1 and HOrF cell lines using SYBER green-based qPCR. Results: The findings demonstrated a significant increase in the cell viability and the proliferation rate after transfection of OECM-1 cells with miR-145 inhibitor. In addition, miR-145 inhibitor transfected cells enhanced C-Myc gene expression and decreased Caspase-3 gene expression OECM-1. Conclusion: Suppression of miR-145 caused a higher proliferation rate in Human Oral Squamous Cell Carcinoma (OECM-1) by increasing C-Myc gene expression and downregulating the level of Caspase-3 gene. | ||
| Keywords | ||
| Keywords: Oral squamous cell carcinoma; MiR-145; C-Myc; Caspase-3 | ||
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