Investigation of the toxoplasmosis prevalence in hemodialysis and kidney transplant patients in the north-west of Iran | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 28 September 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2024.307700.2105 | ||
| Authors | ||
| Abbas Farzin1; Shahram Khademvatan* 1; Hamed Fakhim2; Khadije Makhdoumi3; Akbar Hoseinnejad4, 5 | ||
| 1Department of Parasitology and Mycology, School of Medicine, Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran | ||
| 2Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Department of Internal Medicine, School of Medicine Nephrology and Kidney Transplant Research Center, Clinical Research Institute Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran | ||
| 4Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| 5Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| Abstract | ||
| Background: This study aimed to determine the prevalence of toxoplasmosis using the serological method (ELISA), diagnosing the positive Toxoplasma gondii (T. gondii) cases with the molecular method and comparing the ELISA and PCR methods in hemodialysis and kidney transplant patients in North-West of Iran. Methods: The present cross-sectional study was performed on 180 patients, including 60 transplant patients, 60 hemodialysis patients, and 60 healthy people. Serum IgG and IgM anti-Toxoplasma antibodies were measured using the ELISA method. Molecular diagnosis was performed by PCR on seropositive samples. Results: among 180 participants, 92 were men (51.12%), and 88 were women (48.88%). The results showed that the prevalence of toxoplasmosis has no significant relationship with sex, residence, occupation, soil contact, and education level (P>0.05). In the transplant, dialysis, and control subgroups, 92 people (51.12%) were IgG-positive. While IgM was positive in only one case of a transplant patient in Uremia, 179 other patients, including hemodialysis patients and other transplant patients, as well as the control group, were negative in Tabriz and Urmia. All the samples that were positive in terms of IgG and IgM titers were negative in the PCR test. Conclusion: The results showed that the prevalence of toxoplasmosis in hemodialysis and transplant patients is high in Tabriz and Urmia. Considering the special conditions of hemodialysis patients and the possibility of reactivation of this parasite from the chronic to the acute phase, it is recommended to take care of health issues along with regular screening for anti-Toxoplasma antibodies. | ||
| Keywords | ||
| Toxoplasma gondii; Hemodialysis; Kidney transplantation; PCR; ELISA | ||
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