Lactoferrin and/or Psyllium Seed Husk as Potential Therapeutics for Induced Ulcerative Colitis in Rats | ||
| International Journal of Theoretical and Applied Research | ||
| Article 1, Volume 3, Issue 2, December 2024, Pages 404-415 PDF (694.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ijtar.2024.273165.1076 | ||
| Authors | ||
| Aya F Elmasry* 1; Maha Ghazi Soliman2; Hanaa Abdelfattah Elsaied3; Wedad Abdallah Hassan4; Sara Mohamed Saber Mohamed5; Shimaa Attia Atta6 | ||
| 1Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt. | ||
| 2Prof. of Immunology, Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt. | ||
| 3Prof. of Medical Microbiology, Department of Pharmacology, National Organization for Drug Control and Research, Egyptian Drug Authority (EDA), Cairo, Egypt. | ||
| 4Prof. of Physiology, Department of Pharmacology, National Organization for Drug Control and Research, Egyptian Drug Authority (EDA), Cairo, Egypt. | ||
| 5Associate Professor of Histology and Cell Biology Histology, Department, Egyptian Drug Authority (EDA), Cairo, Egypt. | ||
| 6Associate Professor of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt. | ||
| Abstract | ||
| Ulcerative colitis is a pathological condition characterized by recurrent colon inflammation, causing symptoms such as diarrhea, nausea and abdominal discomfort. In vitro, studies have demonstrated that Lactoferrin directly impacts intestinal immunity and reduces inflammation by altering the production of inflammatory cytokines in immune cells. Plantago ovata's seed husk, known as Psyllium, are frequently taken as a dietary supplement to help with digestive problems. The study investigated the induction of the colitis model in rats using a 1ml of acetic acid (4%) enema inserted into the rectum. Oral Lactoferrin with a dose of (30 mg/animal/day) and Psyllium seed husk colloid with a dose of (15 mg/animal/day) for 7 days were used as treatments. Animals were divided into seven groups with different treatments. Rats' stool was examined and scored. Serum C- reactive protein (CRP), Interleukin-10, and Interleukin-17 were estimated with some oxidative stress parameters evaluated in colon tissue homogenate. Immunohistochemical Interleukin-6 and histological examinations of the colon were assessed. The study found that oral administration of lactoferrin and/or Psyllium significantly improved the severity of colon inflammation by reduction in colon wet-to-dry ratio, colon inflammatory index, serum Interleukin-17, protein expression of Interleukin-6 and CRP, Colon homogenate malondialdehyde (MDA) and myeloperoxidase (MPO) activity. Also, increased serum Interleukin-10 and Glutathione (GSH) levels were determined. The inflamed colons treated with Lactoferrin and/or Psyllium showed a mostly normal histopathological examination with minimal erosion. The present study revealed that Lactoferrin and/or Psyllium prospered to decrease levels of colonic and systemic inflammation in rats-induced colitis, hopefully postponing disease progression. | ||
| Keywords | ||
| Ulcerative Colitis; Lactoferrin; Psyllium seed husk | ||
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