Study the Association of Helicobacter pylori and some of HepatitisC Virus Patients in Egypt | ||
| Egyptian Journal of Microbiology | ||
| Article 3, Volume 52, Issue 1, December 2017, Pages 29-37 PDF (695.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejm.2017.677.1013 | ||
| Authors | ||
| Soheir Abd El-Salam* 1; Marwa Darwish2; Waleed Elagawy3 | ||
| 1Botany Department, Faculty of Science, Benha University, Benha, Egypt. | ||
| 2Biochemistry Department,Faculty of Science, Suez University, Egypt | ||
| 3Department of Tropical Medicine, National Hepatology and Tropical Medicine Research Institute Cairo, Egypt | ||
| Abstract | ||
| BOTH hepatitis C virus and Helicobacter pylori infections are commonly found in Egypt. Correlation among Helicobacter pylori and HCV has been assisted. This work aim was to research H. pylori DNA inside the liver tissue of Egyptian suffers with persistent hepatitis C and find the relation among HP invasion and HCV. This prospective study was conducted with 49 participants. Helicobacter pylori Standard Kit (H.pylori) genome is designed for the in vitro H.pylori genomes from liver tissues patients with chronic hepatitis C quantitatively. A TaqMan® principle was used by the primer and probe. During PCR amplification, primers of bidirectional hybridize to the H.pylori DNA. DNA probe was Labeled with a 5`-dye and a 3`-quencher forms the fluorogenic probe. During PCR amplification, the indicator dye and the quencher are separated and the probe is split. The increase in fluorescence can be detected by PCR platforms. The bacterial DNA was existed in the liver specimens approximately 44.9% of patients. The DNA of bacteria in hepatic tissue was highly recurrent in patients with progressed fibrosis (54.5% vs. 45.5 %, P = 0.02). Meanwhile, the infective dose of HCV was higher in sufferers with HP DNA in liver tissue compared to patients with no HP DNA in liver tissue (9.0×105 vs. 0.05×105). There hasn't been a relation among the tested bacteria and some factors as age, sex and (LFT) liver function tests while (AFP) α-fetoprotein levels did not differ between patients in absence or presence H.pylori DNA. Conclusion: There was coexistence of HP infection, HCV infective dose and liver fibrosis. | ||
| Keywords | ||
| HP DNA; HCV; fibrosis; liver function; PCR | ||
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