Expression of Matrix Metalloproteinase-9, P53 and B-cl2 Genes in Human Osteosarcoma Cell Line Treated with Ginger Extract | ||
| Ain Shams Dental Journal | ||
| Volume 35, Issue 3, September 2024, Pages 232-243 PDF (1.28 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2024.299648.1338 | ||
| Authors | ||
| Hend Mohamed Ali* 1; Enas Alaa Eldin Abd El-Aziz2; Aly Fahmy Mohamed3; Sabreen Gamal Khalil Amar4 | ||
| 1Demonstrator of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Egypt. | ||
| 2Associate Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt. | ||
| 3Former Head of Research and Development Sector of The Egyptian Holding Company of Vaccines, Sera and Drug (Egy-Vac, VACSERA-Egypt). | ||
| 4Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt. | ||
| Abstract | ||
| Aim: The current study was designed to explore the potential effect of ginger extract on the osteosarcoma cell line (MG-63). Material and Methods: Human osteosarcoma cell line (MG-63) was acquired from Nawah-Scientific. The tested drug was ginger extract prepared by maceration in semi-liquid form. The potential impact of ginger extract upon MG-63 cells was evaluated using cytotoxicity assay, microscopic evaluation, Enzyme-Linked Immunosorbent Assay for evaluation of Matrix Metalloproteinase-9 (MMP-9), and Real-Time Polymerase Chain Reaction for detection of the expression levels of the pro-apoptotic gene (P53) and antiapoptotic gene B-cell lymphoma 2 (B-cl2) in ginger extract-treated cells. Then the data collected was statistically assessed. Results: The outcomes demonstrated that ginger extract's cytotoxic impact upon MG-63 cells was dose-dependent, as viability increased as long as the concentration decreased. Under the microscope, MG-63 cells treated with ginger extract displayed apoptotic signs such as chromatin condensation, cell shrinkage, and plasma membrane blebbing. Data about MMP-9 showed that, ginger extract dramatically and dose-dependently reduced MMP-9 levels. Regarding P53 and B-cl2, data revealed that ginger extract significantly increased P53 levels and decreased B-cl2 in a dose-dependent manner. Conclusion: Ginger extract substantially diminishes the viability of osteosarcoma cells because of its strong cytotoxic activity against MG-63 cells. | ||
| Keywords | ||
| Ginger extract; osteosarcoma; apoptosis; P53 and B-cl2 | ||
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