Comparative Effect of Atomoxetine versus Formoterol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male Mice | ||
| Fayoum University Medical Journal | ||
| Volume 14, Issue 1, October 2024, Pages 94-103 PDF (1.26 M) | ||
| Document Type: Review Articles | ||
| DOI: 10.21608/fumj.2024.269849.1323 | ||
| Author | ||
| heba Essam Rashad* | ||
| Histology and cell biology department,Faculty of medicine,Fayoum university ,Fayoum city | ||
| Abstract | ||
| Atrophy of skeletal muscles is still one of numerous diseases' clinical problems. Formetrol, an agonist of the B adrenergic receptor, may prevent this atrophy. An FDA-approved inhibitor of reuptake of norepinephrine called atomoxetine was effective in preventing skeletal muscle atrophy. Aim of work: This study aimed to compare the effect of atomoxetine versus formetrol on dexamethasone-induced skeletal muscle atrophy in male mice. Material and methods: Forty-eight adult male albino mice were divided into six groups (8 mice each): Group I (control group) Group 2 (dexamethasone treated group), Group 3 (atomoxetine only treated group), Group 4 (atomoxetine + dexamethasone treated group), Group 5 (formetrol only treated group) Group 6 (formetrol + dexamethasone treated group).Sections were stained with hematoxylin and eosin stain & Picro Sirius red (PSR) histochemical reaction. Immunohistochemical reaction was done using nuclear factor kappa-B (NF-KB) and heat shock protein (Hsp70) antibodies. The area percentage of collagen fibers deposition, area percent of nuclear factor kappa-B immunoexpression, area percentage of heat shock protein 70 immunoexpression and the diameter of muscle fiber were measured by comp image analaysis. Results: Group 2 (dexamethasone treated group) showed decrease in diameter of muscle fibers. Group 4 (atomoxetine and dexamethasone treated group) and Group 6 (formetrol and dexamethasone treated group) showed increase in diameter of muscle fibers as compared to dexamethasone group. Conclusion: Formetrol (β2-AR) treatment induced skeletal muscle hypertrophy while, atomoxetine doesnot stimulate skeletal muscle hypertrophy, so it has a potential in the prevention of skeletal muscle atrophy. | ||
| Keywords | ||
| Skeletal muscle atrophy; dexamethasone; atomoxetine and formetrol | ||
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