Gomaa, L., Zein, H., Abdel Ghaffar, N., Abdallah, N. (2017). Molecular studies on Isoprene Production from Natural and Recombinant Bacteria. test, 8(2), 65-69. doi: 10.21608/jacb.2017.38435
Lamis Gomaa; Haggag Zein; Nahed Abdel Ghaffar; Naglaa Abdallah. "Molecular studies on Isoprene Production from Natural and Recombinant Bacteria". test, 8, 2, 2017, 65-69. doi: 10.21608/jacb.2017.38435
Gomaa, L., Zein, H., Abdel Ghaffar, N., Abdallah, N. (2017). 'Molecular studies on Isoprene Production from Natural and Recombinant Bacteria', test, 8(2), pp. 65-69. doi: 10.21608/jacb.2017.38435
Gomaa, L., Zein, H., Abdel Ghaffar, N., Abdallah, N. Molecular studies on Isoprene Production from Natural and Recombinant Bacteria. test, 2017; 8(2): 65-69. doi: 10.21608/jacb.2017.38435

Molecular studies on Isoprene Production from Natural and Recombinant Bacteria

Journal of Agricultural Chemistry and Biotechnology
Article 6, Volume 8, Issue 2, February 2017, Pages 65-69    PDF (492.39 K)
Document Type: Original Article
DOI: 10.21608/jacb.2017.38435
Authors
Lamis Gomaa1; Haggag Zein2; Nahed Abdel Ghaffar1; Naglaa Abdallah2
1Agricultural Genetic Engineering Research Institute, ARC, Giza, 12619, Egypt
2Department of Genetics, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Abstract
The natural volatile hydrocarbon isoprene (2-methyl-l, 3-butadiene) is an essential chemical for polymers, drugs and rubber industry. It is produced naturally by animals, plants and bacteria. The isoprene synthase gene was identified in plants. However, it was not identified in bacteria. Moreover, it was found that Bacillus sp.produced the most isoprene compared to other bacteria species. In this work, the quantitative analysis of isoprene produced by Bacillus subtilis ATCC 6633 and Bacillus stearothermophilus 12980 ATCC was measured using gas chromatography-mass spectrometry (GC-MS) compared to standard isoprene, in which retention time was 3.61 for isoprene standard, 3.69 for B. stearothermophilus and 3.68 for B. subtilis. Additionally, Bacillus stearothermophilus 12980 ATCC produced 1.169 mg/ml isoprene, when incubated at 55oC for 18h and Bacillus subtilis 6633 ATCC produced 1.071 mg/ml isoprene when incubated at 45oC for 18h. The synthetic kudzu isoprene synthase gene was used to transform the E. coli DH5α cells.  SDS-PAGE of proteins from the transformed E. coli cells showed a band in the predicted size of the kIspS protein at 65 Kd. In conclusion, from the previous work further studies might develop recombinant bacteria as a biological source capable of isoprene production for chemical feedstock and rubber synthesis. 
Keywords
Isoprene; Isoprene synthase; Bacillus subtilis; Bacillus stearothermophilus
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