Chemical composition, antioxidant, and cytotoxic potential of essential oils of some cultivated plants in Egypt | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 08 October 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.317876.10339 | ||
| Authors | ||
| Mohammed Ibrahim Mohammed Ali1; Ahmed Ragab Hamed* 2; Emad Hassan3; Sayed Abdel Hamid El-Toumy4; samy mostafa1; Faten abou-Elella5; Ahmed Mahmoud Aboul-Enein5 | ||
| 1Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, Egypt | ||
| 2Chemistry of Medicinal Plants Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, Egypt | ||
| 3Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, Egypt | ||
| 4Chemistry of Tannins Department, National Research Centre, Giza, Egypt | ||
| 5Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt | ||
| Abstract | ||
| This study aimed to identify chemical the composition of essential oils (EOs) of Cymbopogon citratus, Lavandula dentata, Artemisia abrotanum, and Laurus nobilis, and evaluate their antioxidant and cytotoxic activities. Methods: The chemical composition investigated by GC/MS, while antioxidant activity evaluated by DPPH and ABTS+ assays. The cytotoxic effect evaluated by MTT technique against human cancer cells; breast (MDA-MB-231), lung (A549), colon (Caco2), liver (HepG2) and doxorubicin-resistant liver (HepG2/DOX). Flow cytometry was utilized to evaluate apoptosis and cell cycle arrest after treating Caco2 cells with EO of C. citratus. Results: The most prominent compound of EOs was citral in C. citratus, artemisia ketone in A. abrotanum and eucalyptol in L. dentata and L. nobilis. The EOs showed weak antioxidant activity; except the EO of L. nobilis which had moderate ABTS+ activity. Also, the EOs exhibited a very weak cytotoxic effect; except the EO of C. citratus showed a high cytotoxic effect against Caco2 and MDA-MB-231, and moderate cytotoxic effect against A549, HepG2 and HepG2/DOX. Cell cycle analysis revealed that the proportion of treated cells reduced in the G0/G1 and G2/M phases, and increased in the S phase. On the other hand, the EO of C. citratus reduces the number of viable and necrotic cells, and increases the number of early and late apoptotic cells. Conclusion: The results revealed that EO of L. nobilis had moderate ABTS+ activity and the EO of C. citratus showed high cytotoxic effect. Flow cytometry showed the EO of C. citrate significantly induces apoptosis and S phase arrest. | ||
| Keywords | ||
| Essential oils; Cytotoxicity; Antioxidant; Medicinal plants; Cell cycle; Apoptosis | ||
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