Bacteriological ad Pathological studies On Mycoplasma gallisepticum in birds | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 61, Issue 4, October 2015, Pages 29-38 PDF (3.67 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2015.385480 | ||
| Authors | ||
| S Attia* 1; Mahmoud M2; A Seida1; Abdelrahman S1 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University | ||
| 2Department of Pathology, Faculty of Veterinary Medicine, Cairo University | ||
| Abstract | ||
| Mycoplasma gallisepticum (MG) is the most Important pathogen of avian Mycoplasmosis, in the present study One hundred and fifty positive serum samples for MG plate agglutination test (SPA) were collected /Vhe included WUNples were one dry Old chicken (36 sample ), broilers (40 sample ), layers (38 sample ), Balady chicken (@4 sample )and turkeys (22 sample). The samples were culltivated ino mycoplasma medium containing sterile filtrated swine serum to detect MG. All suspectcd colonies were identified by conventional method, growth inhibition test and PCI In this studies culture methods detect MG from 35 and 23.35 percent of the suspected samples and Polymerase chain reaction confirmed the nuclele acid of the MG using universal and MG specific primers. Cumulatively PCR confirmed MG positive in (16%) of samples from 150 field samples collected Curing (he study. Tissue samples from the positive MG serological cases were examined histo pathologically for Showing lesions in the tissues. The clinical signs and pathologie lesions were much more significant in the diseased bird by MG. The pathological changes in upper respiratory (ract and lung showing large masses of caseous exudate in the air sacs, predominantly in the abdominal ones and Cpy, Peritonitis was observed in combined infection with CB. Thoracic airsacs. Bilateral thoracic as well as abdominal air saculitis with caseous exudate were observed in severely affected combined cases. | ||
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