Comparison of the ‘influence of egg yolk of different avian species on cryopreservation of canine semen | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 61, Issue 4, October 2015, Pages 121-127 PDF (1.92 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2015.385491 | ||
| Authors | ||
| Salama Ali* 1; Ziada S2; Abdou S1 | ||
| 1Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University | ||
| 2Animal Reproduction Research Institute, Giza | ||
| Abstract | ||
| Egg yolk is one of the most widely used as a constant components for sperm preservation and a wide range of factors affect ‘ts action on sperm motility, viability and fertilizing ability. The aim of this experiment was Co determine the effect of egg yolk from different species, namely the domestic chicken, goose, duck and ostrich on sperm quality following cryopreservation of dog semen. Ejaculates were collected using digital manipulation from five fertile dog. Semen samples were extended with a Tris-citric acid-fructose extender containing the different avian egg yolk (20%) and glycerol (7°C). Straws were equilibrated at 4 °C for 2h, frozen in liquid nitrogen vapor for 10 min and stored in liquid nitrogen (-196 °C). After thawing (37 °C for 30 s), sperm motility, viability, abnormal acrosome and membrane integrity (HOST) were evaluated. Results showed that the duck egg yolk have the best cryoprotective effect ‘n terms of the highest (p < 0.05) post thaw motility and viability percentage (60.00+0.30'% and 161.00+5.05, respectively) compared to the other avian egg yolks. The percentage of acrosomal abnormalities after thawing was significantly (p < 0.05) the lowest (19.20+0.41°%) and sperm membrane integrity and alkaline phosphatase enzyme concentration were the highest (78.00+0.89% and 23,33+1.181U/cm3, respectively) in the duck egg yolk compared to other species. Results suggested that duck egg yolk could be used as an alternative for chicken egg yolk, in a semen extender for the cryopreservation of dog semen, but it requires further evaluation in fertility trials | ||
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