Mesenchymal Stem Cell-Derived Exosomes Versus its Conditioned Medium in Amelioration of Hippocampal Rat Model of Alzheimer's Disease, Behavioral, Biochemical, Histological and Immunohistochemical Study | ||
| Journal of Medical Histology | ||
| Article 2, Volume 7, Issue 2, December 2023, Pages 144-184 PDF (33.26 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2024.296814.1129 | ||
| Authors | ||
| Dina Abdel Dayem* 1; Amira Ahmed2; Walaa Abdelzaher3; Marwa Hamid4; Fatma Khalil5; Nashwa El-Tahawy6; Seham Abd El-Aleem2; Sara Abdel Hafez2 | ||
| 1Department of Histology and Cell Biology, Minia University, Minia | ||
| 21Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, | ||
| 3Department of Medical Pharmacology, Faculty of Medicine, Minia University, Minia | ||
| 4Department of Biochemistry, Faculty of Veterinary, Cairo University, Cairo, | ||
| 5Department of Animal and Poultry Management and Wealth Development, Faculty of Veterinary, Beni-Suef University, Beni-Suef, Egypt | ||
| 6Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, | ||
| Abstract | ||
| Introduction: Alzheimer's disease is the most common form of dementia. Recently, mesenchymal stem cells-conditioned medium and exosomes have lately been employed in several therapeutic investigations. Aim of work: To demonstrate hippocampus’s biochemical, histological and immunohistochemical changes in albino rats after induction of Alzheimer and to evaluate the role of mesenchymal stem cells-conditioned medium versus exosomes on the albino rat Alzheimer model. Materials and Methods: Thirty-two rats were used in this study plus ten rats were used in the preparation of mesenchymal stem cells derived conditioned media and exosomes. Rats were divided into four groups; Control, Alzheimer group, Conditioned media and Exosome treated group. Behavioral tests were performed. The hippocampi were prepared for biochemical, histological and immunohistochemical study. Results: The Alzheimer group showed various behavioral changes. Rats also showed significant changes in hippocampal malondialdehyde, tumor necrotic factor, total antioxidant capacity, acetylcholine esterase and monoamine oxidase. Microscopically, hippocampi showed degenerated neurons as well as argyrophilic neurofibrillary tangles and amyloid plaque formation. The conditioned medium and exosomes treated groups displayed biochemical, functional and structural improvement with reduced argyrophilic neurofibrillary tangles and amyloid plaque formation. Treatment with exosomes was superior to conditioned medium as they improved neuronal function, regulated astrocyte and microglial activities and enhanced regenerative changes. Conclusions: The conditioned medium and exosomes minimized Alzheimer - hippocampal associated biochemical, histological and immunohistochemical degenerative changes with better effect with exosome treatment. These results may pave the future for using exosomes as an effective adjuvant therapy in Alzheimer patients. | ||
| Keywords | ||
| Alzheimer's disease; conditioned medium; hippocampus; mesenchymal stem cell-derived exosomes; neurogenesis | ||
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