Possible Protective Effects of Quercetin versus Quercetin Loaded Chitosan Nanoparticles on Zinc Sulfate Induced Olfactory Mucosa and bulb Injury in Adult Male Albino Rats; Behavioral, Biochemical, Histological and immunohistochemical study | ||
| Journal of Medical Histology | ||
| Article 3, Volume 7, Issue 2, December 2023, Pages 185-218 PDF (27.23 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2024.297558.1130 | ||
| Authors | ||
| Aliaa AbdelHaleem* 1; Amira Ahmed1; Randa Ibrahim1; Sayed EL Sheikh2; Walaa Abdelzaher3; Azza` Ali4; Sara Abdel Hafez4 | ||
| 1Department of Histology and Cell BiologyوFaculty of Medicine, Minia University, Minia, Egypt | ||
| 2Department of Anatomy and Embryology, Faculty of Medicine, Minia University, Minia, Egypt | ||
| 3Department of Medical Pharmacology, Faculty of Medicine, Minia University, Minia, Egypt | ||
| 4Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, Egypt | ||
| Abstract | ||
| Background: Anosmia is a common annoying problem that affects quality of life. Nanoparticles act as nanocarriers that deliver potent drug payloads at a controlled rate at the target site. Purpose: This current work aimed to evaluate the potential preventive benefits of Quercetin (QU) versus Quercetin-loaded chitosan nanoparticles (QU-CSNPs) on an experimental rat model of anosmia and shed insight on its protective mechanisms. Methods: Forty rats were randomly divided into four groups, the Control group, the Zinc sulfate (ZnS) group, the ZnS+QU group, and the ZnS+QU-CSNPs group. Behavioral assessment was carried out. Olfactory mucosae and bulbs were collected for biochemical, histological, immunohistochemical, and morphometric analysis. Results: The ZnS group showed behavioral alternation, decreased glutathione levels, increased malonaldehyde, and tumor necrosis factor-α levels. Olfactory mucosa and bulb showed histological alterations, as well as a significant increase in COX- 2 immune-expression and decrease in PCNA and SOX2 expression. The ZnS+QU-CSNPs group showed amelioration in previously mentioned parameters and histological alternation than the ZnS+QU group. Conclusions: QU-CSNPs has prophylactic effects in an anosmia model than QU via its anti-inflammatory and antioxidant effects. This could pave the way for additional studies to investigate further mechanisms underlying the potential beneficial effects of QU-CSNPs and bring up a new line for anosmia therapy. | ||
| Keywords | ||
| Olfactory Mucosa; quercetin; quercetin Loaded Chitosan Nanoparticles; zinc sulfate | ||
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