Gene Expression Analysis and Anti-cancer Activity of Cyclophosphamide for MCF-7 and H9 Cancer Cell Lines | ||
| Egyptian Journal of Histology | ||
| Article 18, Volume 48, Issue 1, March 2025, Pages 281-292 PDF (682.32 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.313634.2125 | ||
| Authors | ||
| Aymen Radhi Habeeb1; Hamid AL-tameemi* 2; Talib Waseen Hussein1 | ||
| 1Directorate of education of Rasafa , Ministry of education, Iraq | ||
| 2University of Bilad Alrafidain, Baqubah, Iraq | ||
| Abstract | ||
| Introduction: Cancer is a major health problem worldwide, and it is one of the leading causes of death. Preventing tumor development is a major challenge due to the similarity in cellular mechanisms between cancerous and normal cells; however, when using biological substances have high efficiency against cancerous cells and low toxicity against normal cells associated with a good prognosis. Therefore, the current study is designed to study the role of Anise extract (AE) and Anise extract- Chitosan nanoparticles in the synergistic effect with cisplatin in treatment of tumor-induced in female mice. Materials and Methods: Five groups of female mice, 6 mice for each group were included to achieve the goal of the present study. These study groups were control, tumor-induced, Cisplatin treated, Cisplatin – Anise extract treated, and Cisplatin- Anise extract- Chitosan nanoparticles treated groups. The tumor size and some biomarkers (IL-33, LRG-1, and CRP) were considered as indicators for tumor development and response to the treatment protocol. Results: The present study found that the tumor size in the tumor-induced group reach to 33.1 mm after 30 days of inducement, while the tumor size in Cisplatin treated group was significantly smaller than the tumor-induced group. The tumor size in Cisplatin- Anise extract group was smaller than Cisplatin treated group. Also, the effect of cisplatin against a tumor increased when combining anise extract with Chitosan nanoparticles. Due to biomarkers showed IL-33, LRG-1, and CRP levels were higher in tumor group than control and other study groups. Conclusion: The current study concluded that the anise extract has high effectiveness in reinforcement Cisplatin action against tumor development and at the same time increased their effect by nanoparticles. | ||
| Keywords | ||
| Anise extract; biomarkers; cispaltin; nanoparticles; tumor | ||
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