High-throughput Sequencing for differentially expressed microRNAs in Hepatocellular Carcinoma | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 13 November 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.325959.10579 | ||
| Authors | ||
| Noha Eltaweel1; Wafaa Ezzat* 2; Ahmed Heiba2; Ahmed Abdelhalim3; Yosra H. Mahmoud4; Maii Mahmoud Temraz2; Khalda Amr1 | ||
| 1Medical Molecular Genetics dept., NRC, Egypt. | ||
| 2Internal Medicine dept., NRC, Egypt. | ||
| 3Hepatology Department, National Hepatology and Tropical Medicine Research Institute. | ||
| 4Clinical Pathology Department, National Hepatology and Tropical Medicine Research Institute | ||
| Abstract | ||
| Background: HCC's has low survival rate this is due to its late-stage diagnosis. Early diagnosis of cancer liver could be successfully managed with high cure rate exceeding 80%. We aimed to search for the top expressed miRNAs that contribute to the pathogenesis in HCC. Furthermore, we performed Target gene prediction and functional annotation of the miRNAs to identify the functional role of the reported miRNAs. Results: The top DEMs in HCC vs controls were hsa-let-7i-5p, miR-103-3p, miR-24-3p, miR-27a-3p, miR-423-5p and miR-27b-3p. The functional enrichment of those genes revealed the highly enriched KEGG pathways and GO terms. Conclusion: this study showed the top deregulated miRNAs in plasma of HCC patients, their target genes that might contribute in HCC pathway so they might be highly involved in the disease pathogenesis. Further functional studies are crucial to assess their functions and actual target genes. | ||
| Keywords | ||
| HCC; miRNAs; high; throughput; functional annotation | ||
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