Effect of adding butylated hydroxytoluene on post-thaw semen quality of Saanen bucks | ||
| Egyptian Journal of Sheep and Goats Sciences | ||
| Volume 19, Issue 3, December 2024, Pages 5-14 PDF (966.29 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejsgs.2024.338370.1014 | ||
| Author | ||
| Ahmed Seioudy* | ||
| Animal Production Research Institute, Agricultural Research Center, Giza, Egypt | ||
| Abstract | ||
| This study aimed to evaluate the beneficial effect of different concentrations of butylated hydroxytoluene (BHT) as an antioxidant at 0.6, 2.0, and 5.0 mM in Tris-citric acid-glucose (TCG) extender on semen quality of Saanen bucks. During mating season (November-January), four Saanen bucks were used in this study. Following collection, semen was divided into two parts. The first part was diluted with a ratio of 1:5 in TCG solution and then washed twice by centrifugation at 3000 rpm/10 minutes. While, the second part was left unwashed. Semen parts were re-suspended in TCG medium as follows: washed semen, washed semen +0.6 mM BHT, washed semen +2.0 mM BHT, washed semen +5.0 mM BHT, unwashed semen and unwashed semen +5.0 mM BHT. Semen straws were chilled at 4°C for 2 hours and then stored in liquid nitrogen. After thawing, total and progressive sperm motility (%), and dead spermatozoa (%) were recorded by computer-assisted semen analysis (CASA). The obtained results showed that addition of 2.0 mM BHT in washed semen recorded the highest percentages of total and progressive sperm motility and the lowest percentage of dead spermatozoa. The washed semen was significantly (P<0.05) higher compared to unwashed semen. In conclusion, BHT increased significantly (P<0.05) both sperm motility and viability percentages in Saanen buck semen. Also, it could be concluded that, addition of 2.0 mM of BHT was the best concentration to improve semen quality and sperm cryosurvival of Saanen buck semen, by acting as an antioxidant thereby reducing the lipid peroxidation of the sperm. | ||
| Keywords | ||
| Saanen buck; semen; cryopreservation; butylated hydroxytoluene | ||
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