Ashry, H., Mahmoud, A., ’ز Aboelhassan, A., Yasser, N., Bassiony, D., Nagah, A., Hussein, M., Fawzy, M., Ibrahim, H., Ahmed, D., Abdelfattah, E. (2024). The impact of L-DOPA on insect-based waste management tool. test, 20(3), 1-16. doi: 10.21608/ajbs.2024.393582
Hamid Ashry; Aya H. Mahmoud; Aya ’ز Aboelhassan; Nada Yasser; Dyaa Bassiony; Abdalla Nagah; Mohammed Y. Hussein; Mohamed A. Fawzy; Habiba M. Ibrahim; Dina H A. Ahmed; Eman A. Abdelfattah. "The impact of L-DOPA on insect-based waste management tool". test, 20, 3, 2024, 1-16. doi: 10.21608/ajbs.2024.393582
Ashry, H., Mahmoud, A., ’ز Aboelhassan, A., Yasser, N., Bassiony, D., Nagah, A., Hussein, M., Fawzy, M., Ibrahim, H., Ahmed, D., Abdelfattah, E. (2024). 'The impact of L-DOPA on insect-based waste management tool', test, 20(3), pp. 1-16. doi: 10.21608/ajbs.2024.393582
Ashry, H., Mahmoud, A., ’ز Aboelhassan, A., Yasser, N., Bassiony, D., Nagah, A., Hussein, M., Fawzy, M., Ibrahim, H., Ahmed, D., Abdelfattah, E. The impact of L-DOPA on insect-based waste management tool. test, 2024; 20(3): 1-16. doi: 10.21608/ajbs.2024.393582

The impact of L-DOPA on insect-based waste management tool

African Journal of Biological Sciences
Volume 20, Issue 3, December 2024, Pages 1-16    PDF (373.54 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2024.393582
Authors
Hamid Ashry1; Aya H. Mahmoud1; Aya ’ز Aboelhassan1; Nada Yasser1; Dyaa Bassiony1; Abdalla Nagah1; Mohammed Y. Hussein1; Mohamed A. Fawzy1; Habiba M. Ibrahim1; Dina H A. Ahmed2; Eman A. Abdelfattah* 2
1Industrial Entomology approaches project Funded by ASRT
2Entomology Department, Faculty of Science, Cairo University, Giza, Egypt
Abstract
The increase in population leads to a rise in consumption and, consequently, a higher generation of wastes. As a result, managing solid wastes has become a major environmental challenge for governments and communities. To tackle this issue, various techniques have been developed worldwide. Insect-based bioconversion is a promising alternative to traditional waste management. The black soldier fly Hermetia illucens is widely used for this purpose. However, organic wastes may contain contaminants, such as phenolics, especially L-DOPA, that could impact the bioconversion efficiency by imposing physiological costs. Therefore, the current study aimed to assess the impact of L-DOPA on H. illucens larvae by exposing them to different concentrations of L-DOPA (0-150 mg/mL). The fifth instar H. illucens larvae’s gut tissues exhibited a variety of oxidative stress parameters such as oxidants (superoxide anion radical (O2•-)), antioxidants (2,2-diphenyl-1-picrylhydrazyl (DPPH), phosphomolybdenum ability, reducing power ability, or total antioxidant assay), oxidative damage products (protein carbonyls), and detoxification enzymes (α-esterase, acetylcholine esterase (AChE)) were measured. The highest levels of O2•-, protein carbonyls, GR, PPO, DPPH, phosphomolybdenum, total antioxidant ability, reducing power, α-esterase, and AChE occurred at 150 mg/mL L-DOPA treatment with the fold of 0.7, 0.6, 2, 0.6, 1.3, 3.9, 0.2, 18.8, 2.8, 0.6, respectively, compared to control levels. Moreover, all oxidative stress parameters and detoxification enzymes were significantly affected by L-DOPA except APOX activity. The results emphasized the toxicity of L-DOPA on H. illucens.
Keywords
Oxidative stress; Black soldier Fly; L-DOPA; Phenolic compounds; Detoxification enzymes; DPPH
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