Molecular detection of Listeria monocytogenes from aborted women | ||
| Journal of Bioscience and Applied Research | ||
| Article 2, Volume 10, Issue 6, December 2024, Pages 1-8 PDF (993.86 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2024.313565.1072 | ||
| Authors | ||
| Rahma majid Kamel* 1; Manar Radhi Shannon Alyassiry2; Hadeel Jabar Neam3; Rashad imad kadhim4; Noor Alhuda Ahmed3 | ||
| 1AL Qadisiyah university college of biotechnology medical department, Iraq | ||
| 2College of Pharmacy/Babylon University, Iraq | ||
| 3AL Qadisiyah university college of biotechnology medical department | ||
| 4AL Qadisiyah university college of biotechnology Department of Agricultural Biotechnology | ||
| Abstract | ||
| Abortion, an involuntary and spontaneous termination of pregnancy, can be influenced by various factors, including potentially unknown ones. Bacterial infections play a significant role in some cases. Listeria monocytogenes, a facultatively pathogenic microorganism, can lead to the serious illness known as listeriosis, a global foodborne disease. This study aimed to assess the presence of Listeria monocytogenes in placental tissue and vaginal swabs of women experiencing abortion through biochemical and molecular analyses. The study was conducted in 2023 in Al-Diwaniyah, Iraq, and 125 samples were collected. The molecular testing involved PCR amplification of the 16S rRNA gene specific to L. monocytogenes. The study yielded 125 bacterial isolates (10.4% of samples), with all L. monocytogenes isolates (100%) containing virulence genes (inlA, plcA, iapA). The detection of L. monocytogenes is a matter of concern and should be considered by healthcare providers for appropriate management. | ||
| Keywords | ||
| L. monocytogenes; inlA; plcA; iap; PCR | ||
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