Antimicrobial Resistance of E. coli Isolated from Broiler Flocks and Slaughterhouses in Batna District, Algeria | ||
| Journal of Applied Veterinary Sciences | ||
| Article 3, Volume 10, Issue 1, January 2025, Pages 22-31 PDF (344.64 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2024.324530.1433 | ||
| Authors | ||
| Hammou Housseyn Abderrahmane1; Randa Benabdallah2; Nouzha Heleili3; Ammar Ayachi* 4; Omar Bennoune4 | ||
| 1Laboratoryof Epidemio-surveillance, Health and Reproduction, Cell Therapy of Domestic and Wild Animals, University of Chahid Bendjedid 3600, El Tare, Algeria | ||
| 2Laboratory of Epidemiology-surveillance, Health, Production & Reproduction, Cell Therapy of Domestic and Wild Animals, Department of Veterinary Sciences, Faculty of Nature and Life Sciences, University of Chadli Bendjedid, 36000. El Tarf, Algeria | ||
| 3Laboratory of Health, Animal Production and Environment (ESPA), Institute of Veterinary and Agricultural Sciences, University of Batna1, Algeria. | ||
| 4Department of Veterinary Sciences, Institute of Veterinary and Agricultural Sciences, University of Batna1, Algeria Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMPC), University of Batna2, Algeria | ||
| Abstract | ||
| In the last few years, the poultry sector in Batna has faced the emergence of atypical diseases. Many professionals suspect unusual viral infections like Newcastle disease, infectious bronchitis, and avian influenza. These diseases are often associated with colibacillosis and salmonellosis, both of which have developed atypical, multi-drug resistance. combat Escherichia (E.) coli superinfections, a study targeted broiler chickens from 80 flocks and two slaughterhouses in the Batna region. Researchers collected organ samples, including neck skin, from both sick animals and those intended for slaughter, isolating 100 E. coli strains—50 from flocks and 50 from slaughterhouses. antibiotic resistance profiles of E. coli isolated from livestock were 62.5% (50/80), revealing moderate resistance rates to several antibiotics. Specifically, resistance was noted for tetracycline (62%), doxycycline (56%), enrofloxacin (44%), ampicillin (46%), amoxicillin (40%), and sulfamethoxazole-trimethoprim (34%). Resistance to colistin showed a lower rate of 20%; amoxicillin-clavulanate and ticarcillin-clavulanate had a resistance of 12% and 8%, respectively. While gentamicin and chloramphenicol had even lower rates at 8% and 6%. Notably, 94% of strains were sensitive to chloramphenicol, and 92% were sensitive to gentamicin. Additionally, 28% of strains were resistant to three antibiotics, and 18% were resistant to four. Escherichia coli isolated from the slaughterhouse was 83.3% (50 / 60) demonstrated higher antibiotic resistance than those from herds. These isolates were resistant to tetracycline, ampicillin, enrofloxacin, and doxycycline, at 82%, 80%, 78%, and 74%, respectively, and had moderate resistance to nalidixic acid, chloramphenicol, sulfamethoxazole-trimethoprim (SXT), and amoxicillin; resistance rates for colistin, gentamicin, and TTC were lower. In terms of multidrug resistance, 42% of the slaughterhouse strains were resistant to five antibiotics, 18% to four, and 14% to two. Nevertheless, these strains remained highly sensitive to colistin and gentamicin. | ||
| Keywords | ||
| Broiler Flocks; E. coli; Multi-Drug-Resistance; Slaughterhouses | ||
| References | ||
|
ABD EL-HACK, M.E., EL-SAADONY, M.T., SALEM, H.M., EL-TAHAN, A.M., SOLIMAN, M.M., YOUSSEF, G.B.A., TAHA, A.E., SOLIMAN, S.M., AHMED, A.E., EL-KOTT, A.F., AL SYAAD, K.M., and SWELUM, A.A., 2022. Alternatives to antibiotics for organic poultry production: types, modes of action and impacts on bird’s health and production. Poultry Science 101 (4):101696. https://doi.org/10.1016/j.psj.2022.101696
ABERKANE, C.A., MESSAÏ, A., MESSAÏ, C.H., and BOUSSAADA, T., 2023. Antimicrobial resistance pattern of avian pathogenic Escherichia coli with detection of extended-spectrum β-lactamase-producing isolates in broilers in east Algeria. Veterinary World 16 (3) : 449. https://doi.org/10.14202/vetworld.2023.449-454
AGABOU, A., LEZZAR, N., OUCHENANE, Z., KHEMISSI, S., SATTA, D., SOTTO, A., LAVIGNE, J.-P., and PANTEL, A., 2016. Clonal relationship between human and avian ciprofloxacin-resistant Escherichia coli isolates in North-Eastern Algeria European Journal Clinical Microbiology & Infectious Diseases 35: 227-2534. https://doi.org/10.1007/s10096-015-2534-3
AGGAD, H., AMMARA, A., Hammoudi, A.H., and KIHAL, M., 2010. Antimicrobial Resistance of Escherichia coli Isolated from Chickens with Colibacillosis. Global Veterinaria 4 (3): 303-306.
AHMED AMMAR, Y.A., MOULAYA, M., BOUZID, R., BENAMEUR, Q., and AGGAD, H., 2017. Bacterial Resistance of Enterobactereacea isolates in Western Algeria. Journal. Applied. Environmental. Biology. Sciences 7(1): 140-145.
AHMED, H.A., and AL-MAHMOOD, OMAR A., 2023. Food Safety Programs that should be Implemented in Slaughterhouses: Review.Journal of Applied Veterinary Sciences, 8 (2): 80 - 88 (April, 2023). https://javs.journals.ekb.eg
AKKARI,H., HELEILI, N., OZGUMUS,B.O., MERRADI,M., REIS,A., AYACHI,A., AKARSU,N., TUFEKCI,E.F., and KILIÇA.O., 2024. Prevalence and molecular characterization of ESBL/pAmpC producing faecal Escherichia coli strains with widespread detection of CTX-M-15 isolated from healthy poultry flocks in Eastern Algeria Microbial Pathogenesis Volume 196, November 2024, 106973 https://doi.org/10.1016/j.micpath.2024.106973AMANCHA, G., CELIS, Y., IRAZABAL, J., FALCONI, M., VILLACIS, K., THEKKUR, P., NAIR, D., PEREZ, F., and VERDONCK, K., 2023. High levels of antimicrobial resistance in Escherichia coli and Salmonella from poultry in Ecuador. Revista Panamericana Salud Publica 47: e 15. https://doi.org/10.26633/RPSP.2023.15
AWAWDEH, L., FORREST, R., TURNI, C., COBBOLD, R., HENNING, J., and GIBSON, J., 2022. Risk Factors Associated with the Carriage of Pathogenic Escherichia coli in Healthy Commercial Meat Chickens in Queensland, Australia. Poultry 1 (2): 94–110. https://doi.org/10.3390/poultry1020009
BARKA, M.S., CHERIF-ANNTAR, A., and BENAMAR, I., 2021. Antimicrobial resistance patterns and transferable traits in Enterobacteriaceae isolates from poultry in Tlemcen, Algeria. African. Journal. Of Clinical and. Experimental. Microbiology 22 (2): 196 - 203. https://doi.org/10.4314/ajcem.v22i2.12
BENAMEUR, Q., GUEMOUR, D., HAMMOUDI, A., AOUDIA, H., AGGAD, H., HUMBLET, M-F., and SAEGERMAN, C., 2014. Antimicrobial Resistance of Escherichia coli Isolated from Chickens in West of Algeria. International Journal of Sciences: Journal of Basic and Applied Research 13, (1): 366-370. http://gssrr.org/index.php?journal
BENKLAOUZ, M.B, AGGAD, H., and BENAMEUR, Q., 2020. Resistance to multiple first-line antibiotics among Escherichia coli from poultry in Western Algeria. Veterinary World 13 (2): 290-295. https://doi.org/10.14202/vetworld.2020.290-295
BOUDJERDA, D., and LAHOUEL, M., 2022. Virulence and antimicrobial resistance of Escherichia coli isolated from chicken meat, beef, and raw milk. Austral Journal of Veterinary Sciences 54 (3): 115-125. http://dx.doi.org/10.4067/S0719-81322022000300115
CIGE, H.Y., OMOTOSHO, O.O., and IBRAHIM, A.M., 2023 :Evaluation of Antibiotic Resistance Pattern of Escherichia Coli and Salmonella Species Isolated from Cloaca of Indigenous Chickens in Live-bird Markets in Marodi Jeh Region, Somalia. Journal of Applied Veterinary Sciences, 8 (2): 44 – 54 (April, 2023) DOI: 10.21608/javs.2023.179843.1200
COURA, F.M., DINIZ, S.A., SILVA, M.X., ARCEBISMO, T.L. M., MINHARRO, S., FEITOSA, A.C. F., LAGE, A.P., KNÖBL, T., MUSSI, J.M.S., and HEINEMANN, M.B., 2017. Phylogenetic Group of Escherichia coli Isolates from Broilers in Brazilian Poultry Slaughterhouse. TheScientific World Journal Volume 2017, Article ID 5898701, 7 . https://doi.org/10.1155/2017/5898701
GHARIB, N.M., EL OKSH, A.S.A., and ABD-EL TWAB A.A., 2023. Prevalence of Multidrug Resistant Shiga Toxin-Producing Escherichia coli in Broiler Chicken. Benha Veterinary Medical Journal 44 (2): 64-69. https://doi.org/10.21608/bvmj.2023.191764.1635
GREGOVA, G., KMETOVA, M., KMET, V., VENGLOVSKY, J., and FEHER, A.R., 2012. Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse. Annals of Agricultural and Environmental Medicine 19 (1): 75-77.
HALFAOUI, Z., MENOUERI, N.M., and BENDALI, L.M., 2017. Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria. Veterinary World 10 (7): 830. https://doi.org/10.14202/vetworld.2017.830-835
HAMMOUDI, A., and HAGGAD, H., 2008. Antibioresistance of Escherichia coli Strains Isolated from Chicken Colibacillosis in Western Algeria.Turkish Journal of Veterinary and Animal Sciences 32 (2) : 123.
HOSSAIN, M MD. K., SHARIFULL ISLAM, MD., SALAH UDDIN, MD., MIJANUR RAHMAN, A. T.M., ASAD UD-DAULA, MD. A.I., RUBAYA, R., BHUIYA, A.A., NUSRAT, MD. A.A., JAHAN, N., LI, J., and ALAM, J., 2023. Isolation, Identification and Genetic Characterization of Antibiotic-Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh. Antibiotics 12: 41. https://doi.org/10.3390/antibiotics12010041
IBRAHIM, R.A., CRYER, T.L., LAFI, S.Q., BASHA, E-A., GOOD, L., and TARAZI, Y.H., 2019. Resistance, gene characterization and the associated risk factors. BioMed Central Veterinary Research 15: 159. https://doi.org/10.1186/s12917-019-1901-1
JAHANTIGH, R., and DIZAJI, E., 2015. Antimicrobial Drug Resistance Pattern of Escherichia coli Isolated from Chickens Farms with Colibacillosis Infection. Open Journal of Medical Microbiology 5 (4): 159-162. http://dx.doi.org/10.4236/ojmm.2015.54019
JASEEM, W.G., and SHAREEF, A.M., 2023. Antibiotic Resistance Patterns of Escherichia coli Isolated from Broiler Chickens with Colibacillosis in Duhok Province Egyptian. Journal. of Veterinary. Sciences. 54 (1): 137-148. https://doi.org/10.21608/ejvs.2022.156403.1383
JAVED, A., MUMTAZ, S., KHALID, M., SIDDIQUE, A., AKHTAR, T., and RAUF, A., 2024. Antimicrobial resistance profiling of Escherichia coli isolated from retailer poultry samples in Azad Jammu and Kashmir. Journal of Population Therapeutics & Clinical Pharmacology 31 (4): 133-143. https://doi.org/10.53555/jptcp.v31i4.5326
KHANAL, T., RAUT, S.B., and PANERU, U., 2016. Study of antibiotic resistance on Escherichia coli in commercial poultry of Nepal. Nepalese Veterinary Journal. 34 : 6-17. https://doi.org/10.3126/nvj.v34i0.22859
KATHAYAT, D., LOKESH, D., RANJIT, S., and RAJASHEKARA, G., 2021. Avian Pathogenic Escherichia coli (APEC): An Overview of Virulence and Pathogenesis Factors, Zoonotic Potential, and Control Strategies. Pathogens 10 (4) : 467. https://doi.org/10.3390/pathogens10040467
KIM, Y.B., YOON, M.Y., WON K.S., YOON, S., and LEE, Y.J., 2020. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli from broiler chickens in Korea. Journal of Applied Poultry Research 29 (4): 1101–1107. https://doi.org/10.1016/j.japr.2020.09.014
LOGUE, C.M., Sherwood, J.S., OLAH, P.A., and ELIJAH, L.M., 2003.The incidence of antimicrobial resistant Salmonella spp. on freshly processed poultry from US Midwestern processing plants. Journal of Applied Microbiology 94 (1): 16-24. https://doi.org/10.1101/2020.04.20.05091 4
LOWENSTINE, L. 1986. Necropsy procedures. In: Clinical avian medicine and surgery. Harrison, G.J., Harrison L.R (Eds). Philadelphia, W. B. Saunders co, pp. 298.
MEGUENNI, N., CHANTELOUP, N., TOURTEREAU, A., ALI AHMED, C., BOUNAR-KECHIH,S., and SCHOULER, C.,2019. Virulence and antibiotic resistance profile of avian Escherichia coli strains isolated from colibacillosis lesions in central of Algeria. Veterinary World 12 (11) :1840-1848. https://www.veterinaryworld.org/Vol.12/November-2019/21.html
MERATI, R., BOUDRA,A., HAMMOUDI, A.H., and AGGAD, H., 2020. Identification and antimicrobial susceptibility of Escherichia coli isolated from broiler chickens affected by colibacillosis in Tiaret. Journal of Preventive Veterinary Medicine 44 (2) :75-80. https://doi.org/10.13041/jpvm.2020.44.2.75
MESSAÏ, C.R., AÏT-OUDHIA, K., KHELEF, D., HAMDI, T.M., CHENOUF, N.S., and MESSAÏ, M.R., 2024. Epidemiology and Antibiotic Resistance Profiles of Avian Pathogenic Escherichia coli Strains Causing Colibacillosis in Broiler Farms in Eastern Algeria. International Journal of Animal Breeding and Genetics 13 (2): 001-006. www.international scholars journals.org © International Scholars Journals
MGAYA, F.X., MATEE, M.I., MUHAIRWA, A.P., and HOZA, A.S., 2021. Occurrence of Multidrug Resistant Escherichia coli in Raw Meat and Cloacal Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania. Antibiotics 2021, 10 (4), 343. https://doi.org/10.3390/antibiotics10040343
MOAWAD, A.A., HOTZEL, H., NEUBAUER, H., EHRICHT, R., MONECKE, S., TOMASO, H., HAFEZ, H.M., ROESLER, U., and EL‑ADAWY, H., 2018. Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli. Gut Pathogens 10 (1) : 39. https://doi.org/10.1186/s13099-018-0266-5
NOENCHAT, P., SRITHONG, T., LERTPIRIYASAKULKIT, S., NHOONOI, C., SAKSANGAWONG, C., and SORNPLANG, P., 2024. Prevalence and antimicrobial resistance profile of Escherichia coli O157:H7 isolated from chickens and chicken meats from local poultry slaughterhouses in Nakhon Ratchasima Province, Thailand. Veterinary Integrative Sciences 22 (3) : 769-785. https://doi.org/10.12982/VIS.2024.051
PARVIN, MST. S., TALUKDER, S., ALI, MD. Y., CHOWDHURY, E.H., RAHMAN, MD. T., and ISLAM, MD. T., 2020.Antimicrobial Resistance Pattern of Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh. Pathogens 9 (6): 420. https://doi.org/10.3390/pathogens9060420
PHIRI, N., MAINDA, G., MUKUMA, M., SINYANGWE, N.N., BANDA, L.J., KWENDA, G., MUONGA, E.M., FLAVIEN, B.N., MWANSA, M., YAMBA, K., MUNYEME, M., and MUMA, J.B., 2020. Antibiotic-resistant Salmonella species and Escherichia coli in broiler chickens from farms, abattoirs, and open markets in selected districts of Zambia. Journal of Epidemiological Research 6 (1): 13. https://doi.org/10.5430/jer.v6n1p13
PILATI, G.V.T., , SALLES, G.B.C., SAVI, B.P., DAHMER, M., MUNIZ, E.C., FILHO, V.B., ELOIS, M.A., SOUZA, D.S.M., and FONGARO,G., 2024. Isolation and Characterization of Escherichia coli from Brazilian Broilers. Microorganisms 12 (7): 1463. https://doi.org/10.3390/microorganisms12071463
QUINN, P.J., MARKEY, B.K., CARTER, M.E., DONNELLY, W.J.C., LEONARD, F.C., and MAGUIRE, D.,2002. Veterinary Microbiology and Microbial Disease. 2nd Edition, A John Wiley & Sons, Ltd., Publication, 912 PP
SCIBERRAS, M., PIPOVÁ, M., REGECOVÁ, I., JEVINOVÁ, P., and DEMJANOVÁ, S., 2019. Antibiotic resistance of Escherichia coli isolated from broiler chickens. Folia Veterinaria 63 (3): 1-8. https://doi.org/10.2478/fv-2019-0021
STANDARDIZATION OF SUSCEPTIBILITY TO THE NATIONAL SCALE HUMAN AND VETERINARY 2014. Algerian network monitoring the resistance of bacteria to antibiotics. With the collaboration of World Health Organization, 7th edition., 192p
TEGEGNE, H., FILIE, K., TOLOSA, T., DEBELO, M., and EJIGU, E., 2024. Isolation, and Identification of Escherichia coli O157:H7 Recovered from Chicken Meat at Addis Ababa Slaughterhouses. Infection and Drug Resistance: 17: 851-863. https://doi.org/10.2147/IDR.S430115
CLSI, 2020. Performance Standards for Antimicrobial Susceptibility Testing. 30thed. CLSI Supplement M100. Wayne, P.A: Clinical and Laboratory Standards Institute.
WIBAWATI, P.A., HARTADI, E.B., KARTIKASARI, M.A., WARDHANA, D.K., and ABDRAMANOV, A., 2023. Prevalence and profile of antimicrobial resistance in Escherichia coli isolated from broiler meat in East Java, Indonesia. International Journal of One Health 9 (1):27. https://doi.org/10.14202/IJOH.2023.27-31
YULISTIANI, R., PRASEPTIANGGA, D., SUPYANI, and SUDIBYA., 2019. Occurrences of Salmonella spp. and Escherichia coli in chicken meat, intestinal contents and rinse water at slaughtering place from traditional market in Surabaya, Indonesia- International Conference on Food Science and Engineering IOP Conf. Series: Materials Science and Engineering IOP Conference Series Materials Science and Engineering vol 633 : 012007. https://doi.org/10.1088/1757-899X/633/1/012007 | ||
|
Statistics Article View: 383 PDF Download: 435 |
||
