EVALUATION OF CRYPTOSPORIDIUM INFECTION IN IMMUNOCOM PROMISED AND IMMUNOCOMPETENT CASES BY STOOL ANALYSIS VERSUS COPRO-ELISA | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 8, Volume 54, Issue 3, December 2024, Pages 407-413 PDF (4.2 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2024.397255 | ||
| Authors | ||
| DINA SALAH NEGM1; SAHAR M. SELIM1; SHAIMAA A. FARAG1; AYMAN A. ABDEL-WAHAB1; NERMIN M. A. Adawy2; AHMED M. ABU-AMER3; DALIA A. SHAFEY1 | ||
| 1Department of Clinical and Molecular Parasitology, Menoufia University, Shebin El Kom, Menoufia Governorate, Egypt | ||
| 2Department of Pediatrics, Menoufia University, Shebin El Kom, Menoufia Governorate, Egypt | ||
| 3Department of Hepatology and Internal Medicine, the National Liver Institute (NLI) | ||
| Abstract | ||
| Cryptosporidium parvum is an opportunistic intracellular apicomplexan parasite that can cause life-threatening diarrhea in immunocompromised patients and self-limited diarrhea in healthy persons. This study screened Cryptosporidium parvum infection among immunocompromised patients versus immunocompetent persons, comparing different diagnostic methods of Cryptos poridium parvum, and to detect the validity of mini FLOTAC apparatus versus the coproantigen ELISA test. A cross-sectional study was carried on two groups: G1:100 immunocompromised patients recruited from the National Liver Institute, Oncology Institute, Renal Dialysis Unit, over four consecutive seasons. G2: 100 immunocompetent apparently healthy persons. Stool samples were divided into two parts, the first part was examined freshly by direct smear and staining methods, some mixed with formalin 10% for FECM, and some was used in mini-flotac tech nique mixed with zinc-sulphate solution. The second part was frozen in (-20) freezer for later ELISA copro-antigen technique. The samples were C. parvum oocyst examined by direct smear, staining (Iodine and/or Modified Ziehl-Neelsen) and Mini-Flotac apparatus versus C. parvum antigen by coproantigen ELISA as a golden standard test. The results showed that Cryptosporidium parvum infection by copro-antigen ELISA test was 25% among all participants (20.5%) in immunocompromised and (4.5%) in immunocompetents respectively. Regarding diagnostic tests, direct smear had the lowest sensitivity (14%) for Cryp tosporidium Parvum detection while the Mini-FLOTAC technique had the highest sensitivity (84%) compared to the copro-antigen ELISA method which was 100%. | ||
| Keywords | ||
| C. parvum; Stool analysis; Mini-FLOTAC test; Copro-antigen ELISA | ||
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