Identification of multidrug-resistant Enterobacteriaceae harboring blaNDM-1gene isolated from surgical site infection at Benha University Hospital | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 29 December 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2024.340858.2376 | ||
| Authors | ||
| Amira E. Ramadan* 1; Mohamed E. Ramadan2; Nora M. Nassar3; Nashwa E. Ahmed4; Riham N. Ragab1 | ||
| 1Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt | ||
| 2Department of general surgery, Faculty of Medicine, Benha University, Egypt | ||
| 3Department of clinical and chemical pathology, Faculty of Medicine, Benha University, Egypt | ||
| 4Department of medical biochemistry and molecular biology, Faculty of Medicine, Benha University, Egypt | ||
| Abstract | ||
| Background: Surgical site infection (SSI) is a major source of morbidity and mortality. It happens within 30 days of a surgical intervention around or at the incision site. Deeper underlying tissues may be involved. In recent years, multidrug-resistant (MDR) organisms are responsible for a growing proportion of illnesses, which present a significant therapeutic challenge. Aim: This study aimed to evaluate the antibiotic susceptibility pattern of Enterobacteriaceae isolated from SSI in Benha University Hospital and detect the blaNDM-1 gene in carbapenem resistant isolates. Methods: A total of 130 wound samples were cultured on blood agar and MacConkey agar media. Strains were identified by standard bacteriological methods and antibiotic sensitivity testing by VITEK 2 compact system, Detection of MBL (metallo-beta-lactamase) producing isolates phenotypically by double disk synergy test (DDST) and detection of blaNDM-1gene in the resistant strains by Conventional PCR. Results: of 120 culture positive wound samples, 53 strains were Enterobacteriaceae (22Klebsiella species, 20 E.coli, 8 Proteus spp and 3 Citrobacter spp), 59% and 30% of Klebsiella spp and E-coli strains respectively were carbapenem resistant, while none of Proteus or Citrobacter spp were carbapenem resistant. Regarding blaNDM-1 detection it was 61.54% and 66.67% for Klebsiella spp and E.coli respectively. Conclusion: NDM-1 gene propagation in Enterobacteriaceae is a worrying threat which necessitates several control measures. | ||
| Keywords | ||
| SSI; MDR; metallo-beta-lactamase; blaNDM-1 | ||
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