ODONTOGENIC/OSTEOGENIC DIFFERENTIATION POTENTIAL OF CONDITIONED MEDIUM DERIVED FROM STEM CELLS FROM EXFOLIATED DECIDUOUS TEETH USING TWO DIFFERENT CENTRIFUGATION-SETTINGS ON PERIODONTAL LIGAMENT STEM CELLS (AN IN VITRO STUDY) | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 30 December 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.332121.2163 | ||
| Authors | ||
| Ahmed Hanafy* 1; Marwa Magdy Saad Abbass2; Riham Aly3; Ghada Nour Eldeen4; Sahar Shawkat5 | ||
| 1Oral Biology Department, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt. | ||
| 2Oral Biology Department, Faculty of Dentistry, Cairo University | ||
| 3Department of Basic Dental Science, National Research Centre, Cairo, Egypt. Stem Cell Laboratory, Center of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt. | ||
| 4Department of Molecular Genetics and Enzymology, National Research Centre, Cairo, Egypt. Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt. | ||
| 5Oral Biology Department, Faculty of Dentistry, Cairo University, Cairo 11553, Egypt. | ||
| Abstract | ||
| Objectives: evaluation of the odontogenic/osteogenic potential of conditioned medium (CM) harvested from stem cells from human exfoliated deciduous teeth (SHEDs) on the human periodontal ligament stem cells (PDLSCs). Methods: PDLSCs were harvested from sound impacted third molar teeth indicated for extraction while SHEDs were isolated from sound deciduous incisors. SHEDs-CM was collected via two different protocols; (1st protocol entailed centrifugation for 4–5 min. at 440 ×g then the supernatant was collected and re-centrifuged for 1 min. at 4°C at 17,400 ×g, while the 2nd protocol implied centrifugation for 5 min. at 1,500 rpm then the supernatant was collected and re-centrifuged for 3 min. at 3,000 rpm). Assessment of differentiation potential of PDLSCs after culturing with SHEDs-CM prepared by the two protocols was done by monitoring the morphological difference of the odontogenic/osteogenic induced cells upon differentiation, tracing the gene profiles of runt-related transcription factor-2, osteopontin, osteocalcin, alkaline phosphatase and collagen 1, and evaluation of mineral deposition using Alizarin Red staining. Results: PDLSCs assumed round morphology upon differentiation, and statistically significant gene upregulation was recorded in groups that used 1st and 2nd protocols contrasted to the control (P < 0.05), in addition, 2nd protocol groups had significant values when compared to 1st protocols (P < 0.001). Moreover, the production of mineralized nodules was distinguished in groups supplemented with SHEDs-CM as detected by Alizarin Red staining. Conclusions: SHEDs-CM prepared by both protocols possesses the capacity to improve the transformation of PDLSCs into odontogenic/osteogenic lineage, nevertheless, the 2nd protocol possesses a more pronounced differentiation potentials when compared to the 1st one. | ||
| Keywords | ||
| Secretome; Stem cells; Odontogenesis; Osteogenesis; cell differentiation | ||
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