Abdelmotagaly, S., Abdellatif, Y., Zein Eldin, A., Abd Elbar, O., Ibrahim, I. (2024). Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm. test, 32(2), 173-182. doi: https://doi.org/10.21608/AJS.2024.270961.1557
Sara Mohamed Abdelmotagaly; Yasmin Abdellatif; Amal F.M. Zein Eldin; Ola Hussein Abd Elbar; Ibrahim seif Eldin Ibrahim. "Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm". test, 32, 2, 2024, 173-182. doi: https://doi.org/10.21608/AJS.2024.270961.1557
Abdelmotagaly, S., Abdellatif, Y., Zein Eldin, A., Abd Elbar, O., Ibrahim, I. (2024). 'Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm', test, 32(2), pp. 173-182. doi: https://doi.org/10.21608/AJS.2024.270961.1557
Abdelmotagaly, S., Abdellatif, Y., Zein Eldin, A., Abd Elbar, O., Ibrahim, I. Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm. test, 2024; 32(2): 173-182. doi: https://doi.org/10.21608/AJS.2024.270961.1557

Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm

Arab Universities Journal of Agricultural Sciences
Volume 32, Issue 2, December 2024, Pages 173-182    PDF (333.44 K)
Document Type: Original Article
DOI: https://doi.org/10.21608/AJS.2024.270961.1557
Authors
Sara Mohamed Abdelmotagaly* 1; Yasmin Abdellatif1; Amal F.M. Zein Eldin2; Ola Hussein Abd Elbar1; Ibrahim seif Eldin Ibrahim1
1Agricultural Botany Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt
2The Central Laboratory for Date Palm Researches and Development, Agricultural Research Center, Giza 12619, Egypt
Abstract
The purpose of the research was to improve the morpho-anatomical and physiological features of somatic embryos (SEs) of date palms in tissue culture media that have the potential to produce green and healthy plantlets. Copper sulfate (CuSO4) and cobalt chloride (CoCl2) were supplemented to date palm maturation medium at various concentrations, separately or combined. Adding CoCl2 to the media of maturation produced the greatest relative growth and differentiation percentages of date palm embryogenic calli, particularly at 0.52 mgL-1. In comparison to the control, when combined treatments were administered alone, the quantity of the produced somatic embryos rose dramatically (17/jar). Total sugars and free amino acids were the highest in SEs grown on a maturation medium with CoCl2 at 0.26 mgL-1. Moreover, total phenols and indoles were picked significantly at their higher levels in the generated SEs that were cultured on a medium enhanced with 0.52 mgL-1. When the maturation medium was administered along with both as a treatment, the antioxidant enzyme, ascorbate peroxidase, reached its maximum activity. Whereas, adding 0.52 mgL-1 of CoCl2 to the culture medium enhanced the activities up to of highest levels of ascorbate peroxidase,  polyphenol oxidase and phenylalanine ammonia-lyase.  
Keywords
Antioxidant enzymes; Biochemical components; Phoenix dactylifera L; Somatic embryogenesis
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