Ibrahim, R., Gabr, H., Atia, F., Hussein, A. (2025). Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation. test, 17(1), 15-25. doi: 10.21608/eajbsz.2025.404827
Ramy B. Ibrahim; Hala Gabr; Fadia M. Atia; Aida A. Hussein. "Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation". test, 17, 1, 2025, 15-25. doi: 10.21608/eajbsz.2025.404827
Ibrahim, R., Gabr, H., Atia, F., Hussein, A. (2025). 'Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation', test, 17(1), pp. 15-25. doi: 10.21608/eajbsz.2025.404827
Ibrahim, R., Gabr, H., Atia, F., Hussein, A. Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation. test, 2025; 17(1): 15-25. doi: 10.21608/eajbsz.2025.404827

Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation

Egyptian Academic Journal of Biological Sciences, B. Zoology
Volume 17, Issue 1, June 2025, Pages 15-25    PDF (915.46 K)
Document Type: Original Article
DOI: 10.21608/eajbsz.2025.404827
Authors
Ramy B. Ibrahim1; Hala Gabr2; Fadia M. Atia3; Aida A. Hussein1
1Zoology department, Faculty of Science, Suez University, Egypt.
2Hematology department, Faculty of Medicine, Cairo University, Egypt.
3Clinical Pathology department Faculty of Medicine, Suez Canal University, Egypt.
Abstract
Aacute myeloid leukemia (AML) is a severe hematological malignancy with a poor prognosis. Although chemotherapy remains the standard treatment, its effectiveness is limited, with high rates of relapse. Stem cell therapy has emerged as a promising alternative, yet its potential for AML prevention and treatment requires further investigation. This study aimed to evaluate the efficacy of bone marrow-derived mesenchymal stem cells (BM-MSCs) in promoting the maturation of AML blasts. Peripheral blood samples were obtained from 39 AML patients for the isolation of blast cells, while BM-MSCs were derived from healthy donors. An in vitro co-culture system was employed to study the interactions between BM-MSCs and AML blasts, assessing the proliferative capacity of BM-MSCs and their effects on leukemia cells (LCs). Flow cytometry was used to evaluate the expression of specific markers before and after co-culture with allogeneic BM-MSCs, focusing on the induction of AML blast maturation. The results demonstrated a significant reduction in the expression of CD13 and CD33 markers in AML blasts following co-culture with BM-MSCs across all samples. Specifically, the expression of CD13 decreased from 70.74% to 5.02%, and CD33 expression was reduced from 69.49% to 5.14%, indicating a marked decline in AML blast populations. In conclusion, the findings suggest that BM-MSCs appear to be effective in inducing the maturation of AML blasts, offering a potential therapeutic strategy for AML management.
Keywords
Acute myeloid leukaemia (AML); Bone marrow derived Mesenchymal stem cells (BM-MSCs); leukemia cells (LCs)
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