X-ray Repair Cross-Complementing group 1 Single-Nucleotide Polymorphism (rs25487): a Risk Factor for Hepatocellular Carcinoma in the Egyptian Population

Shaaban, Menat Allah A.; Saad, Wessam El.; Abdelhamed, Nada A.; Kamal El-Din, Maha M. M.; Mahmoud, Marwa E.

doi:10.21608/eajbsc.2025.405462

	X-ray Repair Cross-Complementing group 1 Single-Nucleotide Polymorphism (rs25487): a Risk Factor for Hepatocellular Carcinoma in the Egyptian Population
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 17, Issue 1, June 2025, Pages 19-30 PDF (621.74 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2025.405462
Authors
Menat Allah A. Shaaban¹; Wessam El. Saad²; Nada A. Abdelhamed²; Maha M. M. Kamal El-Din³; Marwa E. Mahmoud²
¹Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
²Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
³Internal Medicine, Hepatology and Gastroenterology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Abstract
Chronic hepatitis C virus (HCV) infection is a worldwide etiology of hepatocellular carcinoma (HCC) particularly in Egypt. DNA‐repair systems are responsible for maintaining genomic integrity. Base excision repair (BER) is one of the major DNA‐repair pathways. The X‐ray repair cross‐complementing group 1 (XRCC1) protein encoded by XRCC1 gene is a key player in the multistep BER pathway. Deficiency in the repair capacity due to genetic alterations in DNA‐repair genes can lead to genomic instability and increased risk of cancer development. Our study aimed to investigate the association between XRCC1 gene polymorphism (rs25487) (c.28152A>G) and HCC with chronic HCV infection in Egyptian patients. The study was conducted on 35 patients with HCC and 15 patients with HCV related cirrhosis serving as pathological control group. Detection of XRCC1 gene polymorphism (rs25487) (c.28152A>G) was performed by real time polymerase chain reaction (RT-PCR). The study showed higher frequency of XRCC1 (GG, GA) genotypes and increased (G) (allele) frequency in patients with HCC compared to patients with HCV related cirrhosis. However, the difference was statistically non-significant. Further studies on larger samples size are required, with the need to perform DNA sequencing from tissue samples to confirm the association between XRCC1 gene polymorphism (rs25487) and HCV related HCC in Egyptian population.
Keywords
XRCC1; RT-PCR; Hepatocellular Carcinoma

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