El-Nadi, N., Abd el laah, A., Ahmed, A., Ahmed, N. (2019). Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections. test, 23(Issue 1), 1-5. doi: 10.21608/smj.2019.40607
Nada Abde Fattah El-Nadi; Amany A. Abd el laah; Amal Mostafa Ahmed; Noha Samar Ahmed. "Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections". test, 23, Issue 1, 2019, 1-5. doi: 10.21608/smj.2019.40607
El-Nadi, N., Abd el laah, A., Ahmed, A., Ahmed, N. (2019). 'Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections', test, 23(Issue 1), pp. 1-5. doi: 10.21608/smj.2019.40607
El-Nadi, N., Abd el laah, A., Ahmed, A., Ahmed, N. Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections. test, 2019; 23(Issue 1): 1-5. doi: 10.21608/smj.2019.40607

Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections

Sohag Medical Journal
Article 7, Volume 23, Issue 1, January 2019, Pages 1-5    PDF (218.44 K)
Document Type: Original Article
DOI: 10.21608/smj.2019.40607
Authors
Nada Abde Fattah El-Nadi1; Amany A. Abd el laah2; Amal Mostafa Ahmed3; Noha Samar Ahmed4
1Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egyptز
2Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
3Departement of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
4Department of Medical Parasitology, Faculty of Medicine, Sohag University, Sohag, Egypt.
Abstract
Introduction:Intestinal parasitic infections are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases among the disadvantaged population .This study aimed to evaluate Formol-ethyl acetate concentration method in detection of intestinal parasitic infections.
Methodology / Principal Findings: 100fecal samples were collected, preserved and examined by using formol-ethyl acetate concentration method.Formal-ethyl acetate concentration method (FECM) detectedprotozoan infections in55%of the samples while only 8%helminthic infections were detected.
Conclusions/Significance:The concentration techniques recommended for the qualitative diagnosis of intestinal parasites (both helminths and intestinal protozoa) are the formol-ethyl acetate concentration method (FECM). By centrifugation, it leads to the recovery of all protozoa, eggs, larvae, coccidian and microsporidia present; This method have the advantages of being rapid and suitable for fresh or preserved stool, also used for concentrating parasites on which Zinc sulfate floatation has given poor results due to excessive amounts of fats and fatty acids, and for operculated ova of some trematodes and cestodes, the morphology of most parasite is retained for easy identification and it covers most intestinal parasites.
Keywords
intestinal parasitic infections; Formol-ethyl acetate concentration
Supplementary Files
References
  1. Sow, D., Dieng, Y., Haouchine, D., Niang, K., Niang, T., Sylla, K., &Izri, A. (2017). Comparison of Para-SellesBailenger/Kop-Color Fumouze, Para-Selles-Iodésine/Kop-Color II Fumouze diagnostic kits with conventional microscopic methods in identifying intestinal parasitic diseases in Senegal. Journal of Parasitic Diseases, 41(3), 814-822.
  2. Suwansaksri, J., Nithiuthai, S., Wanitkit, V., Soogarun, S., &Palatho, P. (2002). The formol-ether concentration technique for intestinal parasites comparing 0.1 n sodium hydroxide with normal saline preparations. Southeast Asian J Trop Med Public Health, 33, 97-98.
  3. Garcia, L. S. (2016). Diagnostic Medical Parasitology, 6thEdition
  4. WHO (2001). Basic laboratory methods in medical parasitology. Geneva: World Health Organization. pp. 25-85
  5. Cringoli, G., Rinaldi, L., Maurelli, M. P., &Utzinger, J. (2010). FLOTAC: New multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nature Protocols, 5(3), 503-515. 
  6. Knopp, S., Glinz, D., Rinaldi, L., Mohammed, K. A., N’Goran, E. K., Stothard, J. R., &Utzinger, J. (2009). FLOTAC: A promising technique for detecting helminth eggs in human faeces. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103(12), 1190-1194.
  7. Barda BD, Rinaldi L, Ianniello D, Zepherine H, Salvo F., &Sadutshang T. (2013). Mini-FLOTAC, an Innovative Direct Diagnostic Technique for Intestinal Parasitic Infections: Experience from the Field. PLoS Neglected Tropical Diseases 7(8), 2344. 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  1.  
Statistics
Article View: 284
PDF Download: 600