A Comparative study on fenugreek seed and its oil's in hyperlipidemic rats | ||
| مجلة الاقتصاد المنزلي | ||
| Volume 41, Issue 1, March 2025, Pages 1-30 PDF (610.22 K) | ||
| Document Type: المقالة الأصلية | ||
| DOI: 10.21608/jhe.2025.406643 | ||
| Authors | ||
| Ashraf Abd El- Aziz Abd El-Megeid1; Sonia Salleh El Marasy1; Shaimaa Ibrahim Mahmoud2 | ||
| 1Department of Nutrition and Food Science. Faculty of Home Economics, Helwan university. Egypt | ||
| 2Graduate Student, Nutrition and Food Science Dept, Faculty of Home Economics, Helwan University, Egypt | ||
| Abstract | ||
| This study aimed to evaluate the effect of different levels of fenugreek seed powder as a protein source, along with its oils, on hyperlipidemic rats. The experiment was conducted using 40 adult male albino rats of the Sprague-Dawley strain, with an average weight of 180 ± 5 g. The rats were divided into two main groups. The first main group (5 rats) was fed a basal diet (BD) and used as a control negative group (-ve). The second main group, consisting of 35 rats, was fed a high-fat diet (HFD) for four weeks to induce hyperlipidemia. The second main group was divided into seven subgroups as follows: One of them (5 rats) was fed on HFD used as the (+ve ) control group. The remaining six subgroups included three groups fed high-fat diets (HFDs) containing powdered germinated fenugreek seeds, supplying half, three-quarters, or the full amount of dietary protein. The last three subgroups were fed a high-fats diet (HFDs) containing oil extracted from germinated fenugreek seeds, in amounts equivalent to the oils present in the germinated fenugreek seeds used in the previous three subgroups. At the end of the experiment (4 weeks), rats were anesthetized with ether before being sacrificed after fasting overnight. Blood samples were collected, allowed to clot, and the serum was separated. The serum lipid profile, including cholesterol, triglycerides, and high-density lipoprotein (HDL-c), was measured. Low-density lipoprotein cholesterol (LDL-c), very low-density lipoprotein cholesterol (VLDL-c), atherogenic index (AI), and coronary risk index (CRI) were calculated. Additionally, kidney function markers (uric acid, urea nitrogen, creatinine) and liver enzymes (aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP)) were determined. The results showed that the high-fat diet (HFD) induced hyperlipidemia, which led to a non-significant change in food intake (FI) but a significant increase in body weight gain (BWG%) compared to the negative control group. The HFDs containing three levels of powdered germinated fenugreek seeds resulted in a significant decrease in BWG%, while did not in the three levels of their oils, as compared to the positive control group. Supplemented HFDs with the three levels of powdered germinated fenugreek seeds and their oils, induced a significant improvement in all parameters, as compared to the positive control group. Meanwhile, the positive groups fed HFDs containing a high level of powdered germinated fenugreek seeds and their oil exhibited the most significant improvement. These findings suggest the therapeutic potential of fenugreek seed powder and oil in managing hyperlipidemia and related complications. | ||
| Keywords | ||
| Fenugreek seeds; Fenugreek seeds oil; Rats; Hyperlipidemia; Lipid profile; Kidney function; and Liver enzymes | ||
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