Role of Quercetin in Ameliorating the Histological Changes in a Rat Model of Acute Lung Injury: Histological and Immunohistochemical Study | ||
| Ain Shams Medical Journal | ||
| Article 11, Volume 75, Issue 4, December 2024, Pages 888-901 PDF (1.59 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asmj.2024.321941.1314 | ||
| Authors | ||
| Nehal Ahmed Dowidar* 1; Samah Kandeel Kandeel2; Nadia Fouad Abo Hassan3; Heba Elsayed Sharaf Eldin3 | ||
| 1Histology and cell biology, faculty of medicine, tanta university | ||
| 2Histology & Cell Biology Department, Faculty of Medicine, Tanta University. | ||
| 3Histology & Cell Biology Department, Faculty of Medicine, Tanta University | ||
| Abstract | ||
| Background: Acute lung injury (ALI) leads to disrupted endothelial and epithelial barriers due to associated acute inflammation. Oleic acid (OA)-induced lung injury is a model for induction of ALI in rats. Quercetin is a flavonoid with anti-inflammatory and antioxidant actions. Aim of the Work: This study was done to evaluate the role of quercetin in ameliorating the histological changes in a rat model of acute lung injury through using histological and immunohistochemical techniques. Materials and Methods: Forty-eight albino rats (200 - 250 grams; 8-9 weeks), were divided into three main groups. Group I (Control group), Group II (ALI-induced group): Eight rats received single injection of 50 μL OA dissolved in 1% BSA via rat tail vein, Group III: Sixteen rats received OA as group II, then divided into Subgroup IIIA& Subgroup IIIB: rats received 10 &50 mg/kg quercetin orally daily for 21 days respectively. Results: ALI-induced group showed thickened interalveolar septae, interstitial& perivascular inflammatory cellular infiltrations, congested blood vessels, disorganized alveoli with intra-alveolar and interstitial hemorrhage, bronchioles with separated epithelial lining, perinuclear vacuolation and their lumen contained exudate, and interstitial areas of homogenous acidophilia at H&E sections. Besides, significant increase in the mean area percentage of CD68 positive cells. Also, prominent ultrastructural changes of type I&II pneumocytes, and pulmonary blood capillaries. Quercetin treated groups showed dose-dependent improvement in the histological structure of the lung. Conclusion: Quercetin ameliorates ALI in rats in a dose dependent manner | ||
| Keywords | ||
| ALI; CD68; Electron Microscopy; Oleic Acid; Quercetin | ||
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