Gene amplification and overexpression of Bacillus subtilis L-asparaginase | ||
| Egyptian Pharmaceutical Journal | ||
| Volume 19, Issue 1, January 2020, Pages 25-28 PDF (400.8 K) | ||
| DOI: 10.4103/epj.epj_38_19 | ||
| Authors | ||
| Wafaa K. Hegazy; Mohamed S. Abdel-Salam; Maysa E. Moharam | ||
| Abstract | ||
| Background and objectives The aim of this paper was to focus on improving production level of l-asparaginase by developing recombinant strains. Materials and methods gene was cloned into the shuttle vector pNW33N, and the recombinant plasmid was used to transform protoplast. gene was expressed into both and Enzyme activity of the recombinant strains was measured as compared with wild-type strains. Results gene was successfully subcloned into the recombinant plasmid named S-ASP-NRC-27. The gene was expressed efficiently in both host strains: and . The enzyme activity of the transformants was increased up to threefold under control of Z promoter. Conclusion From the previous results, the shuttle vector pNW33N seemed to be a very useful plasmid as a cloning vector in a wide variety of the genus . Both of gene amplification and the control of Z promoter had direct effects on producing the super -expression strains. | ||
| Keywords | ||
| antitumor; cloning; L-asparaginase | ||
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