Red-pigment production by TRP–NRC from soybean mill via solid-state fermentation | ||
| Egyptian Pharmaceutical Journal | ||
| Volume 21, Issue 1, January 2022, Pages 17-24 PDF (573.13 K) | ||
| DOI: 10.4103/epj.epj_63_21 | ||
| Authors | ||
| Mohamed Fadel; Yomna A.M. Elkhateeb | ||
| Abstract | ||
| Background Color of food is important to appear its freshness, safety, good processing esthetic, and nutritional values. In the recent years, production of biopigments from natural sources as an important alternative to harmful synthetic dyes is of worldwide interest. Objective This study focused on the production of red pigment from local isolated fungus TRP–NRC by cultivation and optimization of production on soybean mill under solid-state fermentation system. Materials and methods A novel local non-mycotoxin-producing fungus TRP–NRC, which is isolated from Egyptian compost and deposited in the gene bank under accession number MW282329, was grown on some agricultural wastes producing industrially red pigment under solid-state fermentation system. About 250-ml conical flasks containing 5 g of substrate, moistened with moisten solution to demand level. The flasks were sterilized, inoculated, and incubated under static condition at 30°C. Studies were conducted for optimization pigment-production conditions, Solid: moisture ratio was tested, 2% of the different carbon sources and equivalent level of nitrogen mg/g substrate were examined. Various pH was adjusted. The effect of incubation temperature was carried out over a range from 20 to 35°C, and the effect of incubation period on pigment production was studied over a range of 5–14 days. Red pigment was extracted from fermented soybean mill with 90% ethanol, solvent was removed using a rotary vacuum evaporator. Estimation of the extracted pigments was done by measuring the absorbance by spectrophotometer OD value at 500 nm. Results and conclusions A novel local non-mycotoxin-producing fungus TRP–NRC was grown on some agricultural wastes for producing red pigment (0.129 g/g soybean original substrate) that were achieved under solid-state fermentation system. The highest production yield of red pigment was reached where the initial pH value was 2.5, temperature 25°C after 7-day incubation when the fungus cultivated in fermentation vessel occupied by 2% w/v soybean mill moistened at solid : liquid ratio 1 : 3. Production of red biopigment shows the advantage of mannitol supplementation as carbon source with soybean for increasing pigment by fold. Pigment production was affected positively by addition of organic nitrogen, especially yeast extract higher than addition of inorganic sources and NHHSO more suitable than the other tested sources after 7 days. High yield of red pigment (mg/g utilizable soybean) makes the production more economical and encourages to be useful in industrial application. | ||
| Keywords | ||
| agricultural wastes; Red pigment; solid-state fermentation system; Talaromyces atroroseus TRP–NRC | ||
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