Ahmed, R., Mwaheb, M., Elmahdi, M., Khamiss Abd Elguaad, M., Eldosoki, D., Mohamed, E., Helal, A., Gaber, S. (2023). The effect of ascorbic acid on histopathological, biochemical, pharmacological, and immunological toxicity of chronic lead acetate exposure on the spleen in a rat model. test, 22(1), 129-142. doi: 10.4103/epj.epj_164_22
Radwa M. Ahmed; Marwa A. Mwaheb; Mohamed H. Elmahdi; Mohamed M. Khamiss Abd Elguaad; Doaa E. Eldosoki; Esam A. Mohamed; Ayman Helal; Sylvana N. Gaber. "The effect of ascorbic acid on histopathological, biochemical, pharmacological, and immunological toxicity of chronic lead acetate exposure on the spleen in a rat model". test, 22, 1, 2023, 129-142. doi: 10.4103/epj.epj_164_22
Ahmed, R., Mwaheb, M., Elmahdi, M., Khamiss Abd Elguaad, M., Eldosoki, D., Mohamed, E., Helal, A., Gaber, S. (2023). 'The effect of ascorbic acid on histopathological, biochemical, pharmacological, and immunological toxicity of chronic lead acetate exposure on the spleen in a rat model', test, 22(1), pp. 129-142. doi: 10.4103/epj.epj_164_22
Ahmed, R., Mwaheb, M., Elmahdi, M., Khamiss Abd Elguaad, M., Eldosoki, D., Mohamed, E., Helal, A., Gaber, S. The effect of ascorbic acid on histopathological, biochemical, pharmacological, and immunological toxicity of chronic lead acetate exposure on the spleen in a rat model. test, 2023; 22(1): 129-142. doi: 10.4103/epj.epj_164_22

The effect of ascorbic acid on histopathological, biochemical, pharmacological, and immunological toxicity of chronic lead acetate exposure on the spleen in a rat model

Egyptian Pharmaceutical Journal
Volume 22, Issue 1, January 2023, Pages 129-142    PDF (15.21 M)
DOI: 10.4103/epj.epj_164_22
Authors
Radwa M. Ahmed; Marwa A. Mwaheb; Mohamed H. Elmahdi; Mohamed M. Khamiss Abd Elguaad; Doaa E. Eldosoki; Esam A. Mohamed; Ayman Helal; Sylvana N. Gaber
Abstract
Objective
To evaluate the effect of vitamin C on histopathological, biochemical, and immunotoxicity of chronic lead exposure in the spleen of a rat model.
Methods
The rats were divided into five groups of 10 rats each: group I received normal saline orally as a control group; groups II and III received lead acetate for 4 and 8 weeks, respectively; and groups IV and V received lead acetate and vitamin C for 4 and 8 weeks, respectively. The spleen was excised and processed for light, electron microscopic, histopathological, and biochemical analyses. Quantitative assessments of matrix metalloproteinase-2 (MMP-2), MMP-9, interleukin-2 (IL-2), IL-6, and tumor necrosis factor-alpha gene expressions were performed by real-time PCR.
Results
The examination of control and vitamin C with lead acetate supplemented groups revealed normal splenic architecture. In contrast, the spleen of lead-intoxicated groups exhibited degenerative changes in the spleen, with a significantly decreased expression of IL-2, glutathione peroxidase, superoxide dismutase, and hemoglobin (<0.05), with significantly increased proinflammatory cytokine (IL-6 and tumor necrosis factor-alpha) expressions, concomitantly with increased oxidative products (malondialdehyde) and protease enzymes (MMP-2 and MMP-9) in the spleen tissues. The coadministration of vitamin C with lead for 4 weeks markedly resolved these changes.
Conclusion
This study may specify the efficiency of vitamin C in lead toxicity prevention in the spleen, represented by the reduced splenic harmful changes produced by lead administration.
Keywords
Histopathological; Immunotoxicity; Lead acetate; spleen; vitamin c
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