Effects of extract on 6-hydroxydopamine neurotoxicity in differentiated SH-SY5Y cells | ||
| Egyptian Pharmaceutical Journal | ||
| Volume 22, Issue 2, April 2023, Pages 188-191 PDF (396.59 K) | ||
| DOI: 10.4103/epj.epj_180_22 | ||
| Authors | ||
| Baris Bitmez; Seda K. Gultekin; Irem G. Albayrak; Yigit Deveci; Yusuf Sicak; Emine Akalin; Adami F. Pirhan; Ulas Gurer; Belkis A. Arslan | ||
| Abstract | ||
| Background and objective Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease. In our study, PD model was created as a result of exposure to 6-hydroxydopamine (6-OHDA) in SH-SY5Y cells, which is a human neuroblastoma cell line. The protective effect of on PD was investigated. Materials and methods Phytochemical analysis of extract was performed. Then, SH-SY5Y cells were differentiated using retinoic acid and then administered 6-OHDA neurotoxin. To determine the protective effects of extract, we investigated the changes in the mRNA expression level of caspase-3, total oxidant status, and antioxidant levels in differentiated SH-SY5Y. Results and conclusion According to our results, extract contains glycosides, tannins, flavonoids, and carbohydrates as the major secondary metabolites. extract significantly reduced caspase-3 gene expression against 6-OHDA toxicity in differentiated SH-SY5Y cells. It was found that total oxidant status level increased significantly in the 6-OHDA experimental group compared with the control and experimental groups. It was found that extract has an inhibitory effect on caspase-3 gene expression, which plays an important role in apoptosis. Therfore extract has been shown to have a therapeutic potential against 6-OHDA toxicity. | ||
| Keywords | ||
| 6-hydroxydopamine; Hypericum perforatum; Parkinson’s; Disease; retinoic Acid; SH-SY5Y Cell line | ||
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